中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2001年
3期
161-163
,共3页
燕东亮%刘丽%石缨%刘立忠%谢宝树%冷爱军%曹颖
燕東亮%劉麗%石纓%劉立忠%謝寶樹%冷愛軍%曹穎
연동량%류려%석영%류립충%사보수%랭애군%조영
前列腺肿瘤%癌%基因
前列腺腫瘤%癌%基因
전렬선종류%암%기인
目的 探讨NF-κB与TNF抗前列腺癌作用的关系。方法 合成全硫代修饰的双链寡核苷酸NF-κB抑制剂；培养人前列腺癌细胞PC-3和成纤维细胞L929,体外分析NF-κB抑制剂对TNF细胞毒作用的影响。制作PC-3细胞裸鼠动物模型，瘤块直径达4mm时，肌注含有94个氨基酸的前列腺分泌蛋白（PSP94）和肿瘤坏死因子衍生物11a融合基因的pcDNA-PSP94-TNFαD11a真核表达质粒DNA，50μg/只，给药一次；肌注NF-κB抑制剂5μmol/只，连续给药10d。设生理盐水和环磷酰胺对照组、pcDNA-PSP94和pcDNA-PSP94-TNFαD11a质粒DNA组、NF-κB抑制剂组。结果 以PC-3及L929为靶细胞,NF-κB抑制剂对TNF的细胞毒作用无明显影响。动物实验结果显示,pcDNA-PSP94-TNFαD11a和NF-κB抑制剂联合用药组抑瘤率为36%，是pcDNA-PSP94-TNFαD11a组的1.8倍，pcDNA-PSP94组与pcDNA-PSP94+NF-κB抑制剂组、NF-κB抑制剂组与生理盐水组肿瘤大小均无明显差别。结论 NF-κB抑制剂在体内可明显增强TNF的抗前列腺癌作用。
目的 探討NF-κB與TNF抗前列腺癌作用的關繫。方法 閤成全硫代脩飾的雙鏈寡覈苷痠NF-κB抑製劑；培養人前列腺癌細胞PC-3和成纖維細胞L929,體外分析NF-κB抑製劑對TNF細胞毒作用的影響。製作PC-3細胞裸鼠動物模型，瘤塊直徑達4mm時，肌註含有94箇氨基痠的前列腺分泌蛋白（PSP94）和腫瘤壞死因子衍生物11a融閤基因的pcDNA-PSP94-TNFαD11a真覈錶達質粒DNA，50μg/隻，給藥一次；肌註NF-κB抑製劑5μmol/隻，連續給藥10d。設生理鹽水和環燐酰胺對照組、pcDNA-PSP94和pcDNA-PSP94-TNFαD11a質粒DNA組、NF-κB抑製劑組。結果 以PC-3及L929為靶細胞,NF-κB抑製劑對TNF的細胞毒作用無明顯影響。動物實驗結果顯示,pcDNA-PSP94-TNFαD11a和NF-κB抑製劑聯閤用藥組抑瘤率為36%，是pcDNA-PSP94-TNFαD11a組的1.8倍，pcDNA-PSP94組與pcDNA-PSP94+NF-κB抑製劑組、NF-κB抑製劑組與生理鹽水組腫瘤大小均無明顯差彆。結論 NF-κB抑製劑在體內可明顯增彊TNF的抗前列腺癌作用。
목적 탐토NF-κB여TNF항전렬선암작용적관계。방법 합성전류대수식적쌍련과핵감산NF-κB억제제；배양인전렬선암세포PC-3화성섬유세포L929,체외분석NF-κB억제제대TNF세포독작용적영향。제작PC-3세포라서동물모형，류괴직경체4mm시，기주함유94개안기산적전렬선분비단백（PSP94）화종류배사인자연생물11a융합기인적pcDNA-PSP94-TNFαD11a진핵표체질립DNA，50μg/지，급약일차；기주NF-κB억제제5μmol/지，련속급약10d。설생리염수화배린선알대조조、pcDNA-PSP94화pcDNA-PSP94-TNFαD11a질립DNA조、NF-κB억제제조。결과 이PC-3급L929위파세포,NF-κB억제제대TNF적세포독작용무명현영향。동물실험결과현시,pcDNA-PSP94-TNFαD11a화NF-κB억제제연합용약조억류솔위36%，시pcDNA-PSP94-TNFαD11a조적1.8배，pcDNA-PSP94조여pcDNA-PSP94+NF-κB억제제조、NF-κB억제제조여생리염수조종류대소균무명현차별。결론 NF-κB억제제재체내가명현증강TNF적항전렬선암작용。
Objective To analyse the effect of NF-κB on the cytotoxity of TNFto prostate cancer.Methods Double stranded oligodexynucleotides as decoy cis-elements that blocked the binding of NF-κB to promoter region of targeted genes has been synthesized.The effect of NF-κB on the cytotoxicity of TNF on L929 and prostate cancer cells PC-3 in vitro was studied.pcDNA-PSP94-TNFαD11α plasmid was injected only once to the nude mice when the implanted PC-3 tumor grown to 4 mm and was in combination with NF-κB decoy injected once daily for 10 days.The control groups of saline,cytophosphamide and injected with pcDNA-PSP94,pcDNA-PSP94-TNFαD11α plasmid DNA or NF-κB decoy were constructed.Results NF-κB decoy had no effect on the cytotoxity of TNF to PC-3 cells and L 929 in vitro.The tumor supression rate of pcDNA-PSP94-TNFαD11α+TNF-κB decoy group was 31%,1.8 times higher than the pcDNA-PSP94-TNFαD11α group.There was no significant difference among the groups with only pcDNA-PSP94,pcDNA-PSP94+NF-κB decoy and NF-κB decoy.Conclusions NF-κB decoy could improve the cytotoxity of TNF to prostate cancer in vivo significangly.
