CAJ | 학술논문

目的：探索蝎毒抗癌多肽组分Ⅲ(antineoplastic polypeptide-Ⅲfrom APBMV，AP-Ⅲ)的分离和纯化方法。方法：蝎毒经预处理后，分别采用pharose FF阳离子交换凝胶柱(cm×5 cm交换柱，流速0．8 ml／min，梯度洗脱1 200 min)层析法和Sepharose FF阳离子交换凝胶柱与Sephadex G-50凝胶柱(柱：100cm×5 cm；流速：0．5 ml／min)联合层析法，分离AP-Ⅲ，用HPLC仪检测2种方法分离出的AP-Ⅲ的纯度及产率。结果：单独用Sepharose FF阳离子交换凝胶柱分离，AP-Ⅲ产率为2．24％，纯度为89％。Sephadex G-50凝胶柱与Sepharose FF阳离子交换凝胶柱联用，其产率及纯度分别提高至4．72％和％。结论：Sephadex G-50与Sepharose FF联用可提高AP-Ⅲ的产率及纯度，从而为开发和利用AP-Ⅲ提供了实验依据。
목적：탐색갈독항암다태조분Ⅲ(antineoplastic polypeptide-Ⅲfrom APBMV，AP-Ⅲ)적분리화순화방법。방법：갈독경예처리후，분별채용pharose FF양리자교환응효주(cm×5 cm교환주，류속0．8 ml／min，제도세탈1 200 min)층석법화Sepharose FF양리자교환응효주여Sephadex G-50응효주(주：100cm×5 cm；류속：0．5 ml／min)연합층석법，분리AP-Ⅲ，용HPLC의검측2충방법분리출적AP-Ⅲ적순도급산솔。결과：단독용Sepharose FF양리자교환응효주분리，AP-Ⅲ산솔위2．24％，순도위89％。Sephadex G-50응효주여Sepharose FF양리자교환응효주련용，기산솔급순도분별제고지4．72％화％。결론：Sephadex G-50여Sepharose FF련용가제고AP-Ⅲ적산솔급순도，종이위개발화이용AP-Ⅲ제공료실험의거。
Aim: To explore the methods on isolation and purification of antineoplastic polypeptide-Ⅲ from APBMV (AP-Ⅲ ). Methods: The AP-Ⅲ was isolated from scorpion venom of Buthus martensii karsch by pretreatment, ion-exchange chro-matography (Sepharose FF, column:25 cm × 5 cm, flow rate:0. 8 ml/min, linear gradient: 1 200 min) or coupling gel filtration(Sephadex G-50, column: 100 cm× 5 cm,flow rate:0.5 ml/min) with Sepharose FF chromatography. The purity of AP-Ⅲ andother compositions of scorpion venom were inspected by high performance liquid chromatography. Results: The purification rateand output of AP- Ⅲ were 89% and 2.24 % with ion-exchange chromatography only, but 95 % and 4.72 % with coupling SephadexG-50 gel filtration with Sepharose FF chromatography. Conclusion: The fractionating method of coupling Sephadex G-50 gel filtra-tion with Sepharose FF chromatography is useful for improving the purification rate and output of AP- Ⅲ. The experimental result isthought as an important evidence to make clinical use of AP- Ⅲ in treatment of tumor.