CAJ | 학술논문

对棉铃虫Helicoverpa armigera (Hübner)谷胱甘肽S-转移酶（GSTs）发育期活性进行了跟踪测定。从卵期开始，GSTs活性呈上升趋势，6龄幼虫达到最高，然后逐渐下降，但化蛹初期仍维持较高水平；用培养基混药法研究发现，芸香苷和2-十三烷酮诱导种群5龄时活性即达到峰值。6龄幼虫脂肪体GSTs活性最高，中肠次之，头部和表皮最低。用1-氯-2,4-二硝基苯（CDNB）作底物时，中肠GSTs的Km值最小。5龄幼虫中肠匀浆液中，GSTs活性90%以上集中在细胞液层，在微粒体、线粒体和细胞碎片层的分布均不超过5%；芸香苷诱导种群3龄幼虫微粒体层GSTs活性分布明显比对照种群减少，而上清液层则比对照种群有所增高。
대면령충Helicoverpa armigera (Hübner)곡광감태S-전이매（GSTs）발육기활성진행료근종측정。종란기개시，GSTs활성정상승추세，6령유충체도최고，연후축점하강，단화용초기잉유지교고수평；용배양기혼약법연구발현，예향감화2-십삼완동유도충군5령시활성즉체도봉치。6령유충지방체GSTs활성최고，중장차지，두부화표피최저。용1-록-2,4-이초기분（CDNB）작저물시，중장GSTs적Km치최소。5령유충중장균장액중，GSTs활성90%이상집중재세포액층，재미립체、선립체화세포쇄편층적분포균불초과5%；예향감유도충군3령유충미립체층GSTs활성분포명현비대조충군감소，이상청액층칙비대조충군유소증고。
The Activity of GSTs in different tissues, developmental stages, subcellular fractions and induction by allelochemicals was determined in cotton bollworm, Helicoverpa armigera (Hübner). GSTs activity toward 1-chloro-2,4-dinitrobenzene (CDNB) was detected in the egg. It increased throughout the larval stages and up to the highest in the sixth instar larvae. The activity of GSTs from the larvae fed on artificial diet containing 50 mg/kg rutin or 2-tridecanone reached the peak at the fifth instar. Enzyme activity was found in fat body, midgut, head and cuticle of the sixth instar larvae. The GSTs activity in fat body was the highest in above tissues. The Km values for CDNB conjugation varied with different tissues, and the Km values of GSTs in the midgut was the lowest (8.5 mmol/L). The supernatant fraction possessed more than 90% of total activities of GSTs in the homogenate of midguts of the fifth instar larvae. The GSTs activity in microsome fraction from the third instar larvae induced by rutin was obviously lower than that fed only on artificial diet, but the enzyme activity in supernatant fraction increased.