CAJ | 학술논문

以17β-雌二醇-黄素腺嘌呤二核苷酸结合物为标记物，以葡萄糖氧化酶为全酶，建立了17β-雌二醇脱辅基酶再激活免疫测定系统(17β-E2-ARIS)，完成了两条标准曲线的制作，通过logit代换法对两条标准曲线进行了直线回归。两条标准曲线的拟合度（r）分别为0.9967**和0.9991**，灵敏度分别为11.54 pg*mL-1 （2.31 pg*孔-1）和\{14.88\} pg*mL-1（2.98 pg*孔-1），平均变异系数（CV%）分别为4.64%和9.34%，测定范围为31.25～1000 pg*mL-1。
이17β-자이순-황소선표령이핵감산결합물위표기물，이포도당양화매위전매，건립료17β-자이순탈보기매재격활면역측정계통(17β-E2-ARIS)，완성료량조표준곡선적제작，통과logit대환법대량조표준곡선진행료직선회귀。량조표준곡선적의합도（r）분별위0.9967**화0.9991**，령민도분별위11.54 pg*mL-1 （2.31 pg*공-1）화\{14.88\} pg*mL-1（2.98 pg*공-1），평균변이계수（CV%）분별위4.64%화9.34%，측정범위위31.25～1000 pg*mL-1。
Apoenzyme reactivation immunoassay system for 17β-estradiol was constructed with 17β-estradiol-flavin adenine dinucleotide conjugate as label and with glucose oxidase as holoenzyme。Two standard curves were obtained，and linerized by logitic trasmission。The regression cofficients are 0.9967 and 0.9991（n=7） resp.，sensitivity 11.54 pg*mL-1 （2.31 pg*well-1） and 14.88 pg*mL-1 （2.98 pg*well-1）resp.，mean CV% 4.64 and 9.34 resp.，and the range of determination is from 31.25 to 1 000 pg*mL-1。