CAJ | 학술논문

目的　了解三峡库区重庆段移民区宿主动物携带肾综合征出血热病毒状况和自然疫源地类型，为库区移民卫生防病工作提供依据。方法　进行现场流行病学调查(含流行病学资料，啮齿类生态和病原检测)。应用直接免疫荧光和原位杂交技术分别检测宿主HFRSV-Ag和HFRSV-RNA。结果　在5个移民区宿主带毒率分别为19.4%、17.0%、14.0%、13.7%和8.5%。原位核酸分子杂交检测证实，5个移民区宿主动物均存在HFRSV-RNA。结论　该研究证实这5个移民区为HFRS的自然疫源地，三峡库区成库后水位提升，鼠类将大量迁移，可能造成HFRS暴发或流行，加强移民区灭鼠与防鼠工作十分必要。
목적　료해삼협고구중경단이민구숙주동물휴대신종합정출혈열병독상황화자연역원지류형，위고구이민위생방병공작제공의거。방법　진행현장류행병학조사(함류행병학자료，교치류생태화병원검측)。응용직접면역형광화원위잡교기술분별검측숙주HFRSV-Ag화HFRSV-RNA。결과　재5개이민구숙주대독솔분별위19.4%、17.0%、14.0%、13.7%화8.5%。원위핵산분자잡교검측증실，5개이민구숙주동물균존재HFRSV-RNA。결론　해연구증실저5개이민구위HFRS적자연역원지，삼협고구성고후수위제승，서류장대량천이，가능조성HFRS폭발혹류행，가강이민구멸서여방서공작십분필요。
Objective　To ascertain the natural infection rate of hemorrhagic fever with renal syndrome virus (HFRSV) among its hosts and the type of the natural foci for providing some baseline data for the immigrant health and epidemic prevention of the Three-Gorge region. Methods　Epidemiological survey on the field was performed including epidemiological data collection, ecology of rodents and pathogen detection. HFRS virus antigen of hosts were detected by the direct immunofluorescent assay (DIFA) technique and determination of HFRSV-RNA by ISH were carried out from HFRSV-Ag-positive animals. Results　HFRSV-Ag-positive animals were found in 5 migration areas ie Baitao Town of Fuling Section, Wansheng Village of Fengjie County and Dachang Town of Wushan County. The positive hosts were as follows, Rattus Norvegicus, Apodemus agrarius, Anourusurex squamipes, Mus musculus and Rattus flavipectus. The positive rate of HFRSV in the mice of 5 migration areas were 19.4%, 17.0%, 14.0%, 13.7%, and 8.5% respectively. The results showed that the lung tissues of some hosts in all five migration areas were HFRSV-RNA-positive. Baitao Town and Peishi Town were attributed to mixture type epidemic areas while. Kangle Town, Wansheng Village and Dachang Town were domestic rats type epidemic areas. Conclusion　This study shows that the five migration areas are natural epidemic foci of HFRS. It is predicted that maximum risk of HFRS breakout or epidemic may take place after the completion of the San Xia Reservoir(the Three-Gorges Reservoir), which results from rodent moving toward higher land. Therefore, deratization and preventive measures for rat are important in migration areas.