CAJ | 학술논문

目的研究病毒唑(三氮唑核苷)在细胞培养中抑制呼吸道合胞病毒(RSV)的作用。方法用细菌培养法，采用Vero细胞感染病毒后2h给药法，观察细胞病变(CPE)。结果当病毒感染量小于或等于100TCID50时，病毒唑组无CPE，而病毒对照组有25%～75%的CPE。病毒唑抑制RSV的最大无毒浓度(TD0)，半数有效浓度(IC50)，最小有效浓度(MTC)和治疗指数(TI)分别为100、50、25μg/ml和4。结论病毒唑在细菌培养中确有显著抑制RSV的作用，这为其治疗RSV感染提供了实验依据。
목적연구병독서(삼담서핵감)재세포배양중억제호흡도합포병독(RSV)적작용。방법용세균배양법，채용Vero세포감염병독후2h급약법，관찰세포병변(CPE)。결과당병독감염량소우혹등우100TCID50시，병독서조무CPE，이병독대조조유25%～75%적CPE。병독서억제RSV적최대무독농도(TD0)，반수유효농도(IC50)，최소유효농도(MTC)화치료지수(TI)분별위100、50、25μg/ml화4。결론병독서재세균배양중학유현저억제RSV적작용，저위기치료RSV감염제공료실험의거。
Objective　To study the effect on ribavirin inhibiting RSV in the condition of cell culture.Methods　By the method of cell culture and the way of drug administered after two hours when Vero cell were infected by virus,CPE was observed.Results　When TCID50≤100,there was no any CPE in the group of ribavirin,but there was 25%～75% CPE in the group of viral control.TD0、IC50，MTC and T1 of ribavirin against RSV were 100ug/ml,50ug/ml,25ug/ml and 4,respectively.Conclusions　Ribavirin can inhibit RSV signific antly in cell culture,This study has provided the laboratory evidences that ribavirin used to treat RSV infection.