CAJ | 학술논문

目的了解激光心肌血运重建术时激光对心肌的损伤.方法在狗心肌上用CO2激光打孔.术后即刻、两周时于打孔处取材,用硝基蓝四唑法作琥珀酸脱氢酶、乳酸脱氢酶组织化学染色.结果术后即刻,孔道及其周围心肌分为五个区.中央为孔道即组织汽化区,向外依次为碳化区、酶消失区、酶减少区、酶浓缩区.两周后孔道区被肉芽组织取代,周围心肌细胞酶活性正常.结论激光心肌血运重建术术后即刻,孔道周围心肌细胞酶活性由内向外依次呈消失、减少、浓缩改变.术后两周可逆性损伤的心肌细胞酶活性恢复.
목적료해격광심기혈운중건술시격광대심기적손상.방법재구심기상용CO2격광타공.술후즉각、량주시우타공처취재,용초기람사서법작호박산탈경매、유산탈경매조직화학염색.결과술후즉각,공도급기주위심기분위오개구.중앙위공도즉조직기화구,향외의차위탄화구、매소실구、매감소구、매농축구.량주후공도구피육아조직취대,주위심기세포매활성정상.결론격광심기혈운중건술술후즉각,공도주위심기세포매활성유내향외의차정소실、감소、농축개변.술후량주가역성손상적심기세포매활성회복.
Objective　Laser-induced myocardial injury was investigated in a canine transmyocardial laser revascularization model. Methods　A total of 23 channels were created in the left ventricles of 5 animals with a CO2 laser. Four animals were sacrificed immediately and one at week 2. Lactate dehydrogenase and succinate dehydrogenase in channel regions were examined with a Nitro-BT method staining. Results　Immediately after the procedure, laser channels and surrounding injured myocardium could be stratified from the center to the border into 5 concentric zones, i.e., vaporized zone, carbonized zone, enzyme-disappeared zone, enzyme-decreased zone and enzyme-concentrated zone. Two weeks later, the phenomenon disappeared. Conclusions　Immediately after transmyocardial revascularization, there is a ring of enzyme-concentrated zone at the outermost region of injured myocardium. Two weeks later, enzyme activities of reversibly injured myocytes appear to have recovered.