CAJ | 학술논문

从中国农业大学试验田中生长的健康甜菜肉质根内分离到1株分泌高活性抗真菌色素物质的优势内生细菌TCl，通过形态学、生理生化特征和16SrDNA系统发育分析，确认该菌株为绿针假单胞菌致金色假单胞菌亚种（Pseudomonas chlororaphis subsp．aureofaciens）。对TCI发酵分泌物经硅胶柱层析分离提纯后，采用薄层层析（TLC）和紫外一可见光谱扫描分析确认其主要活性成分为吩嗪一1一羧酸（Phenazine一1一Carboxylic Acid，PCA）。从TCl菌悬液浸种处理过的甜菜苗根内可以重复分离到完全相同的菌株，表明TCl的确可以在甜菜根内定殖，该菌株有可能被制成活菌制剂作为生物农药直接应用于宿主植物病害的生物防治。
종중국농업대학시험전중생장적건강첨채육질근내분리도1주분비고활성항진균색소물질적우세내생세균TCl，통과형태학、생리생화특정화16SrDNA계통발육분석，학인해균주위록침가단포균치금색가단포균아충（Pseudomonas chlororaphis subsp．aureofaciens）。대TCI발효분비물경규효주층석분리제순후，채용박층층석（TLC）화자외일가견광보소묘분석학인기주요활성성분위분진일1일최산（Phenazine일1일Carboxylic Acid，PCA）。종TCl균현액침충처리과적첨채묘근내가이중복분리도완전상동적균주，표명TCl적학가이재첨채근내정식，해균주유가능피제성활균제제작위생물농약직접응용우숙주식물병해적생물방치。
A prevailing endophytic bacterial strain TC1 was isolated from inside beet root grown in an experimental field of China Agricultural University. This strain could excrete a kind of orange substance which had broad-spectrum strong antifungal activity. By the combination of phenotypie profiles, physiological and biochemical characteristics and phylogenetic analysis based on 16S rRNA gene sequences we identified TC1 as Pseudomonas ehlororaphis subsp, aureofaciens. We determined the major component of TC1 excreta as Phenazine-l-carboxylic Acid （PCA） by thin layer chromatogram （TLC） and UV-VIS absorbent spectrum scanning after TCI excreta was separated and purified with silica gel column chromatogram method. After seeds were soaked in suspension of TC1 and planted, the same strain could be isolated from inside roots of beet seedlings repeatedly, indicating that the strain TC1 could colonize inside beet root indeed. The strain TC1 has the potential to be processed into living-bacteria preparation and applied to bio-protection of host plant.