山东化工
山東化工
산동화공
SHANDONG CHEMICAL INDUSTRY
2011年
8期
70-72
,共3页
咸鸭蛋清%亲和层析%抗生物素蛋白%酶联免疫
鹹鴨蛋清%親和層析%抗生物素蛋白%酶聯免疫
함압단청%친화층석%항생물소단백%매련면역
salted duck egg white%affinity chromatography%avidin%enzyme - linked immunosorbent assay
采用2-亚氨基生物素琼脂糖凝胶4B从酸热变性后咸鸭蛋清中亲和分离了抗生物素蛋白，并利用生物素化的过氧化物酶偶联起显色反应，建立了一种简便的抗生物素蛋白含量测定新方法．进而对咸鸭蛋清抗生物素蛋白进行了普查。实验结果表明：咸鸭蛋清抗生物索蛋白回收率达60．1％±5．0％，纯化倍数为222．5，纯化蛋白经SDS—PAGE电泳均显示单一蛋白染色带，其对应的分子量约为67．8K，咸鸭蛋清抗生物素蛋白质量分数约为0．05％，是抗生物索蛋白潜在的资源，本研究为咸鸭蛋清的回收利用打下了基础。
採用2-亞氨基生物素瓊脂糖凝膠4B從痠熱變性後鹹鴨蛋清中親和分離瞭抗生物素蛋白，併利用生物素化的過氧化物酶偶聯起顯色反應，建立瞭一種簡便的抗生物素蛋白含量測定新方法．進而對鹹鴨蛋清抗生物素蛋白進行瞭普查。實驗結果錶明：鹹鴨蛋清抗生物索蛋白迴收率達60．1％±5．0％，純化倍數為222．5，純化蛋白經SDS—PAGE電泳均顯示單一蛋白染色帶，其對應的分子量約為67．8K，鹹鴨蛋清抗生物素蛋白質量分數約為0．05％，是抗生物索蛋白潛在的資源，本研究為鹹鴨蛋清的迴收利用打下瞭基礎。
채용2-아안기생물소경지당응효4B종산열변성후함압단청중친화분리료항생물소단백，병이용생물소화적과양화물매우련기현색반응，건립료일충간편적항생물소단백함량측정신방법．진이대함압단청항생물소단백진행료보사。실험결과표명：함압단청항생물색단백회수솔체60．1％±5．0％，순화배수위222．5，순화단백경SDS—PAGE전영균현시단일단백염색대，기대응적분자량약위67．8K，함압단청항생물소단백질량분수약위0．05％，시항생물색단백잠재적자원，본연구위함압단청적회수이용타하료기출。
Avidin was purified by 2 -imminobiotin -agarose affinity gel chromatography from salted duck eggs white （SDEW）. A measuring method of avidin in SDEW was established by using biotinylated peroxidase to couple its coloring reaction. Then the content of avidin in SDEW was determined. Experimental results showed that avidin was purified to give single protein staining band in SDS - PAGE with 60. 1% recovery and 222. 5 purification folds by 2 - imminobiotin - agarose affinity gel chromatography from salted duck eggs white. The subunit molecular weights of the avidin was 67.8KD. The content of protein in SDEW was determined to be 0.05%. Thus, SDEW was an excellent alternative resource for preparation of avidin. This study laid a foundation for the utilization of the salted duck eggs white waste in future.
