CAJ | 학술논문

[目的]对发情期蓝狐卵巢的组织结构和卵泡发育过程的变化进行研究.[方法]采集10只发情期蓝狐的左侧卵巢共计10枚，利用光学显微镜对卵巢的组织结构和卯泡的发育过程进行观察，同时利用目镜测微尺和显微照相技术分别对30个原始卵泡、30个初级卵泡、15个生长卵泡、15个囊状卵泡、15个成熟卵泡及各级卵泡中的卵母细胞进行测量和拍照。[结果]蓝狐卵巢由生殖上皮、白膜、皮质和髓质构成。皮质内分布着大量的间质细胞及不同发育阶段的卵泡，位于卵巢的外周；髓质位于皮质下层，分布着较多的血管。卵泡发育过程中，各级卵泡和卯母细胞的直径差异较大（卵泡为45．45～974．55μm，卵母细胞为30．23～147．27μm），在初级卵泡阶段出现透明带物质。卵泡闭锁发生于卵泡生长的各个时期，主要表现为卵母细胞的皱缩，细胞核固缩；颗粒细胞松散、退化。[结论]为揭示雌性蓝狐的生殖机理提供了组织学依据。
[목적]대발정기람호란소적조직결구화란포발육과정적변화진행연구.[방법]채집10지발정기람호적좌측란소공계10매，이용광학현미경대란소적조직결구화묘포적발육과정진행관찰，동시이용목경측미척화현미조상기술분별대30개원시란포、30개초급란포、15개생장란포、15개낭상란포、15개성숙란포급각급란포중적란모세포진행측량화박조。[결과]람호란소유생식상피、백막、피질화수질구성。피질내분포착대량적간질세포급불동발육계단적란포，위우란소적외주；수질위우피질하층，분포착교다적혈관。란포발육과정중，각급란포화묘모세포적직경차이교대（란포위45．45～974．55μm，란모세포위30．23～147．27μm），재초급란포계단출현투명대물질。란포폐쇄발생우란포생장적각개시기，주요표현위란모세포적추축，세포핵고축；과립세포송산、퇴화。[결론]위게시자성람호적생식궤리제공료조직학의거。
[Objective] The ovary microstructure and the changes of the follicle during the development period of blue fox during the estrous period were studied.[Method] Ten left ovaries were collected from 10 female blue foxes during the estrous period. The histological structure of the ovary and follicular development process were observed by using optical microscope. And 30 primordial follicles, 30 primary follicles, 15 growing follicles, 15 vesicular follicle, 15 mature follicles and the oocytes in the follicles of different developing stages were measured and photographed by eyepiece micrometer and photomicrographic technique respectively. [Result] The ovary of blue fox consisted of germinal epithelium, tunica albuginea, cortex and medulla. The cor- tex, which was located at the periphery of the ovary, contained many interstitial cells and follicles at different developing stages. Medulla was located below the cortex, with a lot of blood vessels. The diameters of follicles at different developmental stages and oocytes had greater differences during the developmental period of follicle. The diameter of follicles was 45.45-974.55 μm and the diameter of oocytes was 30.23-147.27 μm. The zona pellucida appeared at the primary follicle stage. The atresia of follicles occurred at all the developmental stages of follicle and the main manifestations were the shrunk oocyte and karyon, and loose and degraded granular cells. [Conclusion] The research provided histological basis for revealing the reproductive mechanism of female blue fox.