CAJ | 학술논문

目的探讨大黄素诱导细胞凋亡对大鼠急性胰腺炎(acute pancreatitis,AP)的治疗作用.方法 45只SD大鼠分为对照组、胰腺炎组、治疗组,每组15只.观察胰腺组织内腺泡细胞凋亡指数、NF-κB mRNA、Bax mRNA、外周血中中性粒细胞(polymorphonuclear neutrophii,PMN)凋亡指数及天冬氨酸特异性半胱氨酸蛋白酶(caspase-3、caspase-8)的变化情况.结果与胰腺炎组比较,治疗组腺泡细胞和PMN凋亡指数及胰腺组织内NF-κB mRNA、Bax mRNA、PMN caspase-3与caspase-8表达均明显增加(F=853.199,327.126,143.586,48.857,231.750,96.552,P＜0.05).治疗组中,胰腺组织病理学评分与胰腺腺泡细胞凋亡指数、PMN凋亡指数均呈负相关(r=-0.96,-0.94,P＜0.05);胰腺腺泡细胞凋亡指数与胰腺组织内NF-κB mRNA、Bax mRNA表达呈正相关(r=0.73,0.76,P＜0.05);胰腺组织内NF-κB mRNA与Bax mRNA表达呈正相关(r=0.94,P＜0.05);PMN凋亡指数与PMN caspase-3、caspase-8表达均呈正相关(r=0.99,0.99,P＜0.05).结论大黄素能有效治疗AP,其机制是通过Bax途径诱导胰腺腺泡细胞凋亡,并通过caspase途径来诱导外周血中PMN凋亡,从而明显减轻AP病情程度.
목적 탐토대황소유도세포조망대대서급성이선염(acute pancreatitis,AP)적치료작용.방법 45지SD대서분위대조조、이선염조、치료조,매조15지.관찰이선조직내선포세포조망지수、NF-κB mRNA、Bax mRNA、외주혈중중성립세포(polymorphonuclear neutrophii,PMN)조망지수급천동안산특이성반광안산단백매(caspase-3、caspase-8)적변화정황.결과 여이선염조비교,치료조선포세포화PMN조망지수급이선조직내NF-κB mRNA、Bax mRNA、PMN caspase-3여caspase-8표체균명현증가(F=853.199,327.126,143.586,48.857,231.750,96.552,P＜0.05).치료조중,이선조직병이학평분여이선선포세포조망지수、PMN조망지수균정부상관(r=-0.96,-0.94,P＜0.05);이선선포세포조망지수여이선조직내NF-κB mRNA、Bax mRNA표체정정상관(r=0.73,0.76,P＜0.05);이선조직내NF-κB mRNA여Bax mRNA표체정정상관(r=0.94,P＜0.05);PMN조망지수여PMN caspase-3、caspase-8표체균정정상관(r=0.99,0.99,P＜0.05).결론 대황소능유효치료AP,기궤제시통과Bax도경유도이선선포세포조망,병통과caspase도경래유도외주혈중PMN조망,종이명현감경AP병정정도.
Objective To investigate the efficacy of emodin in the treatment of acute pancreatitis in rats.Methods Forty-five SD rats were divided into control group,pancreatitis group and treatment group.The apoptotic index of pancreatic acinar cells.the expression of NF-κB mRNA and Bax mRNA,the apoptosis rate of peripheral polymorphonuclear neutrophil(PMN),and the expression of PMN caspase-3 and PMN caspase-8 were detected.Results Compared with the panereatitis group,the apoptotic index of pancreatic acinax cells and PMN,and the expressions of NF-κB mRNA,Bax mRNA.PMN caspase-3 and PMN caspase-8 in pancreas tissue were significantly increased in the treatment group(F=853.199,327.126,143.586,48.857,231.750,96.552,P＜0.05).The histopathologic score of panereatitis Was negatively correlated with the apoptotic index of the pancreatic acinar cells and PMN(r=-0.96,-0.94,P＜0.05).The apeptotic index ofthe pancreatic acinar cells Was positively correlated with the expression of NF-κB mRNA,Bax mRNA in pancreas tissue(r=0.73,0.76,P＜0.05).The expression of NF-κB mRNA was positively correlated withthat of Bax mRNA(r=0.94,P＜0.05).The apoptotie index of PMN was positively correlated with the expression of PMN caspase-3 and PMN caspase-8 (r=0.99,0.99.P＜0.05).Conclusions Emodin alleviates acute pancreatitis via regulating the apoptosis of pancreatic acinar cells through Bax pathway,and regulating the PMN apoptosis through caspase pathway.