CAJ | 학술논문

目的观察丰富环境刺激对快速老化小鼠( SAMP8)精神行为学及促肾上腺皮质激素(ACTH)和皮质醇(Cor)水平的影响.方法共选取雄性3月龄SAMP8小鼠20只,采用随机数字表法将其分为丰富环境组和标准环境组,每组10只；同时选取雄性3月龄抗快速老化(SAMR1)小鼠5只纳入对照组.标准环境组及对照组小鼠均给予标准环境饲养,丰富环境组小鼠在饲养过程中给予丰富环境刺激.于饲养2个月后采用自主活动仪和高架十字迷宫检测各组小鼠精神行为学变化情况,同时采用放射免疫方法检测各组小鼠体内ACTH及Cor含量.结果与对照组比较,5月龄SAMP8小鼠自主活动次数及高架十字迷宫中开放臂停留时间均显著减少(P＜0.05)；与标准环境组比较,丰富环境组SAMP8小鼠自主活动次数显著增多(P＜0.05),高架十字迷宫测试时开放臂停留时间延长,但与标准环境组间差异无统计学意义(P＞0.05)；放射免疫检测结果显示,与对照组比较,5月龄SAMP8小鼠体内ACTH及Cor水平均显著降低(P＜0.05)；与标准环境组比较,丰富环境组SAMP8小鼠ACTH水平显著提高(P＜0.05),Cor水平与标准环境组间差异无统计学意义(P＞0.05).结论 5月龄SAMP8小鼠已出现明显精神行为学异常,体内ACTH及Cor含量均较正常水平降低；丰富环境刺激可提高SAMP8小鼠ACTH含量,对减轻精神行为异常具有重要作用.
목적 관찰봉부배경자격대쾌속노화소서( SAMP8)정신행위학급촉신상선피질격소(ACTH)화피질순(Cor)수평적영향.방법 공선취웅성3월령SAMP8소서20지,채용수궤수자표법장기분위봉부배경조화표준배경조,매조10지；동시선취웅성3월령항쾌속노화(SAMR1)소서5지납입대조조.표준배경조급대조조소서균급여표준배경사양,봉부배경조소서재사양과정중급여봉부배경자격.우사양2개월후채용자주활동의화고가십자미궁검측각조소서정신행위학변화정황,동시채용방사면역방법검측각조소서체내ACTH급Cor함량.결과 여대조조비교,5월령SAMP8소서자주활동차수급고가십자미궁중개방비정류시간균현저감소(P＜0.05)；여표준배경조비교,봉부배경조SAMP8소서자주활동차수현저증다(P＜0.05),고가십자미궁측시시개방비정류시간연장,단여표준배경조간차이무통계학의의(P＞0.05)；방사면역검측결과현시,여대조조비교,5월령SAMP8소서체내ACTH급Cor수평균현저강저(P＜0.05)；여표준배경조비교,봉부배경조SAMP8소서ACTH수평현저제고(P＜0.05),Cor수평여표준배경조간차이무통계학의의(P＞0.05).결론 5월령SAMP8소서이출현명현정신행위학이상,체내ACTH급Cor함량균교정상수평강저；봉부배경자격가제고SAMP8소서ACTH함량,대감경정신행위이상구유중요작용.
Objective To investigate the effects of an enriched environment (EE) on the psychological and behavioral symptoms of senescence and on the level of plasmic adrenocorticotropic hormone (ACTH) and serum corticosteroid (Cor). Methods Twenty male senescence-prone SAMP8 mice of 3 months old were divided randomly into an enriched environment (EE) group and a standard environment (SE) group.Five male senescence-resistant SAMR1 mice of the same age served as the control group.Behavioral symptoms were assessed after 2 months using autonomic activity and elevated-plus maze (EPM) test performance.Plasma ACTH and serum Cor were detected using radio-immunologic methods. Results Average autonomic activity frequency and EPM open-arm times were significantly lower with the SAMP8 mice than among the control group,and the autonomic activity frequency was significantly higher in the EE group than in the SE group of SAMP8 mice.The average EPM open-arm times of the EE group and the SE were not significantly different.Average ACTH and Cor levels were both significantly lower in the SAMP8 mice,and the level of ACTH was significantly higher in the EE group than in the SE group.There was no significant difference in these groups' average Cor levels. Conclusions The SAMP8 mice of 5 months demonstrated significant mental and behavioral abnormalities,as well as down-regulated plasma levels of ACTH and Cor.EE An enhanced environment can reduce behavioral disturbance through up-regulating ACTH.