中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2009年
2期
103-106
,共4页
郝牧%孟恒星%李刚%漆佩静%徐燕%李长虹%王亚非%邱录贵
郝牧%孟恆星%李剛%漆珮靜%徐燕%李長虹%王亞非%邱錄貴
학목%맹항성%리강%칠패정%서연%리장홍%왕아비%구록귀
胎血%间质干细胞%造血干细胞%归巢%细胞黏附分子
胎血%間質榦細胞%造血榦細胞%歸巢%細胞黏附分子
태혈%간질간세포%조혈간세포%귀소%세포점부분자
Fetal blood%Mesenchymal stem cells%Hematopoietic stem cell%Homing%Cells adhesion molecules
目的 探讨脐带来源间充质干细胞(MSC)对脐血来源CD34+细胞在NOD/SCID小鼠体内归巢的影响及其可能的机制.方法 将CD34+细胞与MSC细胞共移植入经放射线照射后的NOD/SCID小鼠,采用流式细胞术和RT-PCR检测移植后20 h NOD/SCID小鼠骨髓及脾脏中人CD34+细胞,计算其相应的骨髓和脾脏的归巢效率.将脐血CD34+细胞与脐带MSC体外共培养,检测MSC细胞对CD34+细胞趋化功能的影响;并于培养4、7 d检测培养后CD34+细胞表面CD49e、CD31、CD62L、CD11a等归巢相关黏附分子表达情况.结果 ①移植后20 h采用流式细胞术成功在小鼠骨髓和脾脏中检测到人CD45+细胞.共移植组CD34+细胞骨髓归巢率[(7.2±1.1)%]高于单移植组[(5.4±0.9)%](P<0.05).②RT-PCR结果 显示共移植组小鼠骨髓细胞和脾脏细胞,单移植组小鼠脾脏细胞扩增得到人GAPDH基因片段,而单移植组小鼠骨髓细胞未见明显扩增条带.③MSC存在时,CD34+细胞的体外迁移能力为(35.7±5.8)%,显著高于CD34+细胞自发迁移率[(3.5±0.6)%,P<0.05].④CD34+细胞与MSC体外共培养后细胞表面CD49e、CD31和CD62L黏附分子的表达水平高于CD34+细胞单独培养组.结论 MSC细胞与CD34+细胞共移植有利于CD34+细胞向骨髓、脾脏等造血器官归巢,这可能与MSC促进CD34+细胞迁移以及维持CD34+细胞表面归巢相关黏附分子的表达相关.
目的 探討臍帶來源間充質榦細胞(MSC)對臍血來源CD34+細胞在NOD/SCID小鼠體內歸巢的影響及其可能的機製.方法 將CD34+細胞與MSC細胞共移植入經放射線照射後的NOD/SCID小鼠,採用流式細胞術和RT-PCR檢測移植後20 h NOD/SCID小鼠骨髓及脾髒中人CD34+細胞,計算其相應的骨髓和脾髒的歸巢效率.將臍血CD34+細胞與臍帶MSC體外共培養,檢測MSC細胞對CD34+細胞趨化功能的影響;併于培養4、7 d檢測培養後CD34+細胞錶麵CD49e、CD31、CD62L、CD11a等歸巢相關黏附分子錶達情況.結果 ①移植後20 h採用流式細胞術成功在小鼠骨髓和脾髒中檢測到人CD45+細胞.共移植組CD34+細胞骨髓歸巢率[(7.2±1.1)%]高于單移植組[(5.4±0.9)%](P<0.05).②RT-PCR結果 顯示共移植組小鼠骨髓細胞和脾髒細胞,單移植組小鼠脾髒細胞擴增得到人GAPDH基因片段,而單移植組小鼠骨髓細胞未見明顯擴增條帶.③MSC存在時,CD34+細胞的體外遷移能力為(35.7±5.8)%,顯著高于CD34+細胞自髮遷移率[(3.5±0.6)%,P<0.05].④CD34+細胞與MSC體外共培養後細胞錶麵CD49e、CD31和CD62L黏附分子的錶達水平高于CD34+細胞單獨培養組.結論 MSC細胞與CD34+細胞共移植有利于CD34+細胞嚮骨髓、脾髒等造血器官歸巢,這可能與MSC促進CD34+細胞遷移以及維持CD34+細胞錶麵歸巢相關黏附分子的錶達相關.
목적 탐토제대래원간충질간세포(MSC)대제혈래원CD34+세포재NOD/SCID소서체내귀소적영향급기가능적궤제.방법 장CD34+세포여MSC세포공이식입경방사선조사후적NOD/SCID소서,채용류식세포술화RT-PCR검측이식후20 h NOD/SCID소서골수급비장중인CD34+세포,계산기상응적골수화비장적귀소효솔.장제혈CD34+세포여제대MSC체외공배양,검측MSC세포대CD34+세포추화공능적영향;병우배양4、7 d검측배양후CD34+세포표면CD49e、CD31、CD62L、CD11a등귀소상관점부분자표체정황.결과 ①이식후20 h채용류식세포술성공재소서골수화비장중검측도인CD45+세포.공이식조CD34+세포골수귀소솔[(7.2±1.1)%]고우단이식조[(5.4±0.9)%](P<0.05).②RT-PCR결과 현시공이식조소서골수세포화비장세포,단이식조소서비장세포확증득도인GAPDH기인편단,이단이식조소서골수세포미견명현확증조대.③MSC존재시,CD34+세포적체외천이능력위(35.7±5.8)%,현저고우CD34+세포자발천이솔[(3.5±0.6)%,P<0.05].④CD34+세포여MSC체외공배양후세포표면CD49e、CD31화CD62L점부분자적표체수평고우CD34+세포단독배양조.결론 MSC세포여CD34+세포공이식유리우CD34+세포향골수、비장등조혈기관귀소,저가능여MSC촉진CD34+세포천이이급유지CD34+세포표면귀소상관점부분자적표체상관.
Objective To investigate the effect and the potential mechanism of umbilical cord (UC) derived mesenchymal stem cells (MSCs) on umbilical cord blood(UCB) derived CD34+ cells in vivo homing in xenotransplanted NOD/SCID mice model. Methods CD34+ cells and MSCs were derived from fresh UCB and UC, respectively. CD34+ cells (5×105 per mice) and MSC cells(5×106 per mice) were co-transplan-ted into irradiated NOD/SCID mice intravenously. CD34+ cells(5×105 per mice) alone were transplanted into the mice as control group. CD34+ cells homed in bone marrow and spleen of recipient mice were detected 20 hours after transplant by FACS and RT-PCR, and the homing efficiencies were calculated. The effect of MSCs on CD34+ cells chemotactic fuction was investigated after co-cultured UCB CD34+ cells with UC MSCs in vitro. After 4 and 7 days coculture, the homing rehted adhesion molecules (the CD49e, CD31, CD62L,CD11a)expressed on CD34+ cells were detected by FACS. Results ①The homing efficiencies in bone marrow in experimental and control group were (7.2±1.1) % and (5.4±0.9) %, respectively (P < 0.05). ②Human GAPDH gene was detected in bone marrow in experimental group and in spleen in both groups. ③The migration efficiency of CD34+ cells was significantly higher in experimental group (35.7±5.8) % than in control group (3.5±0.6)% (P <0.05). ④The expression of CD49e,CD31 ,CD62L on CD34+cells kept higher level in MSCs cocuitured group than in CD34+ cells alone group. Conclusions MSCs can efficiently increase homing of CD34+ cells to bone marrow and spleen in vivo by keeping a high level of hom-ing adhesion molecules expression and improving migration efficiency of UCB CD34+ cells.
