CAJ | 학술논문

目的探讨在脱细胞牛心包上种植人血管混合细胞构建组织工程心脏瓣膜的方法与效果.方法将体外培养的人血管混合细胞种植在脱细胞牛心包材料上,观察种子细胞形态学和免疫组织化学变化和牛心包超微结构变化,并测定内皮细胞分泌t-PA和PAI-1的活性.结果种子细胞全层覆盖脱细胞牛心包表面,并浸润至组织内部生长;牛心包表面为扁平、片状、分层的细胞覆盖,细胞表面有少量绒毛和纤细的纤维结构.免疫组化显示内皮细胞Ⅷ因子、平滑肌细胞α-肌动蛋白、成纤细胞Fibronectin相关抗原呈阳性表达;种子细胞能分泌纤溶活性物质t-PA和PAI-1.结论人血管混合细胞种植后附着满意,与脱细胞牛心包生物相容性好,生长状况好,并具有内皮功能.
목적 탐토재탈세포우심포상충식인혈관혼합세포구건조직공정심장판막적방법여효과.방법 장체외배양적인혈관혼합세포충식재탈세포우심포재료상,관찰충자세포형태학화면역조직화학변화화우심포초미결구변화,병측정내피세포분비t-PA화PAI-1적활성.결과 충자세포전층복개탈세포우심포표면,병침윤지조직내부생장;우심포표면위편평、편상、분층적세포복개,세포표면유소량융모화섬세적섬유결구.면역조화현시내피세포Ⅷ인자、평활기세포α-기동단백、성섬세포Fibronectin상관항원정양성표체;충자세포능분비섬용활성물질t-PA화PAI-1.결론 인혈관혼합세포충식후부착만의,여탈세포우심포생물상용성호,생장상황호,병구유내피공능.
Objective To evaluate the method and outcome of mixed human vascular ceils seeding on decellularized bovine pericardium in constructing a tissue engineered heart valves (TEHVs). Methods The mixed human vascular cells obtained by primary co-culture were seeded on decellularized bovine pericardium to construct a TEHVs. The morphologic and immunohistochemistry changes of the seeding cells, the ultramicrostructure of the decellularized bovine pericardium seeded with mixed human vascular cells were observed dynamically. Results The seeded cells overlaid on the deeellularized bovine pericardium gradually, and with the time-lapse, the cells stratified and penetrated into the deep layer of the pericardium. The mixed human vascular cells were verified as endothelial cells (ECs), smooth warble cells (SMC) and fibroblasts by the immunohistochemistry. And the seeding cells were testified for their ability of secreting t-PA and PAI-1 by enzyme-labeled immunosorbent assay (ELISA). The seeding cells on the pericardium were flat, lamellated or inordinate, and then stratified, with some floss and fibre. Conclusion The histocompatibility of the mixed human vascular cells and the decellularized bovine pericardium is satisfactory. The cells can grow well on the pericardium and possessed some functions of ECs.