CAJ | 학술논문

目的建立人类膀胱肿瘤多药耐药(MDR)细胞株并研究其耐药机制.方法以人类膀胱肿瘤细胞株T24为研究对象,用多柔比星(ADM)浓度梯度递增诱导法,建屯人类膀胱肿瘤细胞多药耐药模型(简称T24/ADM).用MTT法检测肿瘤细胞的MDR特性;RT-PCR检测膀胱肿瘤耐药细胞株MDR基因的表达情况.流式细胞术检测耐药细胞P-糖蛋白(P-gp)的表达.结果 T24/ADM对多种化疗药物产生耐药,对ADM的耐药性提高了16.3倍.RT-PCR检测发现T24/ADM中MDR基因和P-gp的表达明显增强.结论 T24/ADM细胞具有MDR特性,其耐药性与MDR基因和P-gp的过表达有关.
목적 건립인류방광종류다약내약(MDR)세포주병연구기내약궤제.방법 이인류방광종류세포주T24위연구대상,용다유비성(ADM)농도제도체증유도법,건둔인류방광종류세포다약내약모형(간칭T24/ADM).용MTT법검측종류세포적MDR특성;RT-PCR검측방광종류내약세포주MDR기인적표체정황.류식세포술검측내약세포P-당단백(P-gp)적표체.결과 T24/ADM대다충화료약물산생내약,대ADM적내약성제고료16.3배.RT-PCR검측발현T24/ADM중MDR기인화P-gp적표체명현증강.결론 T24/ADM세포구유MDR특성,기내약성여MDR기인화P-gp적과표체유관.
Objective To establish a multidrug resistance cell line of human bladder tumor and study its characteristics and mechanism of muhidrug resistance. Methods Human bladder tumor cell line T24 was induced to muhidrug resistance cell line T24/ADM by intermittent administration of high dose ADM. The multidrug resistance to multianticancer agents of the ceils was evaluated by MTT assay; the expression of MDR gene was assessed by RT-PCR and P-gp expression was determined by flow cytometry. Results T24/ ADM resisted to many anti-tumor agents, and its IC50 of ADM was 16.3 times higher than that of T24. Significant overexpression of MDR gene and p-gp of the multidrug resistant cells were detected. Conclusion T24/ADM is a human muhidrug-resistant cell line, and it's drug resistance relates to the overexpression of MDR gene and p-gp.