CAJ | 학술논문

目的研制角质形成细胞-胶原-猪去细胞真皮基质(PADM)组织工程皮肤,观察气-液面培养对角质形成细胞分化及细胞因子基因表达的影响.方法将角质形成细胞种植在真皮基质胶原面进行气-液面培养和浸没式培养.采用逆转录-聚合酶链反应(RT-PCR)技术,对比研究两种培养方法获得的细胞膜片bFGF基因表达差异.结果气-液面培养获得的角质形成细胞层结构与正常皮肤相似,细胞分化良好.单纯浸没式培养角质形成细胞层较薄,分化较差.气一液面培养获得的细胞膜片bFGF mRNA表达量明显高于单纯浸没式培养(t=3.825,P<0.01).结论气-液面培养可促进角质形成细胞的生长、分化及复层表皮结构形成,影响细胞因子的基因表达.
목적 연제각질형성세포-효원-저거세포진피기질(PADM)조직공정피부,관찰기-액면배양대각질형성세포분화급세포인자기인표체적영향.방법 장각질형성세포충식재진피기질효원면진행기-액면배양화침몰식배양.채용역전록-취합매련반응(RT-PCR)기술,대비연구량충배양방법획득적세포막편bFGF기인표체차이.결과 기-액면배양획득적각질형성세포층결구여정상피부상사,세포분화량호.단순침몰식배양각질형성세포층교박,분화교차.기일액면배양획득적세포막편bFGF mRNA표체량명현고우단순침몰식배양(t=3.825,P<0.01).결론 기-액면배양가촉진각질형성세포적생장、분화급복층표피결구형성,영향세포인자적기인표체.
Objective To prepare tissue-engineered skin containing keratinocytes and dermal matrix prepared by Co[[agen-PADM ,and observe the influence of air-liqnid interface culture on differentiation and cytokine gene expression of keratinocytes. Methods The keratinocytes were inoculated on the collagen surface of the dermal matrix by air-liquid interface culture and imergecl culture. By using RT-PCR method,the bFGF gene expression in keratinocytes sheets was compared. Results The structure of keratinocytes by air-liqnid interface culture was similar to that of the normal skin, and keratinocytes differentiated well. By using imerged culture method, the structure of keratinocytes was thin, and keratinocyte differentiation was immature. The UFGF-RNA expression of keratinocytes sheet was higher by air-liquid interface culture (t = 3.825 ,P<0.01). Conclusion Air-liquid interface culture may facilitate the growth, differentiation and multiple stratum of keratinocytes, and influence the cytokine gene expression.