CAJ | 학술논문

目的通过检测转化生长因子-β1(TGF-β1)在大鼠膝关节内侧副韧带(MCL)损伤模型中的表达以及观察局部瘢痕愈合的情况,探讨脉冲超声波治疗对韧带损伤愈合的作用.方法选择18只Sprague-Dawley大鼠,行内侧副韧带切断术,造模成功后随机分为对照组、0.5 W/cm~2超声组和1.0 W/cm~2超声组.对照组不予以治疗,0.5 W/cm~2超声组和1.0 W/cm~2超声组依次予以每天10 min相应强度的脉冲超声波治疗,治疗8 d后处死各组大鼠.大体观察各组大鼠局部韧带愈合的情况后,取内侧副韧带进行苏木素-伊红(HE)染色、Van Gieson(VG)染色及免疫组织化学染色,观察片比较各组大鼠炎症细胞浸润、胶原纤维排列情况及TGF-β1表达强度.结果 ①肉眼观察:断裂的内侧副韧带均以瘢痕组织形式愈合,与对照组比较,2个超声组的瘢痕组织明显增大.②HE染色以及VG染色后光镜下观察:对照组中炎症细胞以及新生血管数较2个超声组少,并且对照组胶原纤维排列较0.5 W/cm~2超声组、1.0 W/cm~2超声组紊乱.③0.5 W/cm~2超声组、1.0 W/cm~2超声组的TGF-β1表达较对照组强,差异有统计学意义(P<0.05);1.0 W/cm~2超声组的TGF-β1表达较0.5 W/cm~2超声组强,差异有统计学意义(P<0.05).结论 ①脉冲超声波治疗韧带损伤,可使局部瘢痕组织增生,炎症反应增强,胶原纤维排列规则,从而改善近期软组织愈合,远期效果尚待进一步研究.②软组织损伤后,脉冲超声波治疗可以促进TGF-β1表达增加;并且治疗强度增大,TGF-β1表达增强.③脉冲超声波治疗可能通过促进TGF-β1表达而促进软组织愈合.
목적 통과검측전화생장인자-β1(TGF-β1)재대서슬관절내측부인대(MCL)손상모형중적표체이급관찰국부반흔유합적정황,탐토맥충초성파치료대인대손상유합적작용.방법 선택18지Sprague-Dawley대서,행내측부인대절단술,조모성공후수궤분위대조조、0.5 W/cm~2초성조화1.0 W/cm~2초성조.대조조불여이치료,0.5 W/cm~2초성조화1.0 W/cm~2초성조의차여이매천10 min상응강도적맥충초성파치료,치료8 d후처사각조대서.대체관찰각조대서국부인대유합적정황후,취내측부인대진행소목소-이홍(HE)염색、Van Gieson(VG)염색급면역조직화학염색,관찰편비교각조대서염증세포침윤、효원섬유배렬정황급TGF-β1표체강도.결과 ①육안관찰:단렬적내측부인대균이반흔조직형식유합,여대조조비교,2개초성조적반흔조직명현증대.②HE염색이급VG염색후광경하관찰:대조조중염증세포이급신생혈관수교2개초성조소,병차대조조효원섬유배렬교0.5 W/cm~2초성조、1.0 W/cm~2초성조문란.③0.5 W/cm~2초성조、1.0 W/cm~2초성조적TGF-β1표체교대조조강,차이유통계학의의(P<0.05);1.0 W/cm~2초성조적TGF-β1표체교0.5 W/cm~2초성조강,차이유통계학의의(P<0.05).결론 ①맥충초성파치료인대손상,가사국부반흔조직증생,염증반응증강,효원섬유배렬규칙,종이개선근기연조직유합,원기효과상대진일보연구.②연조직손상후,맥충초성파치료가이촉진TGF-β1표체증가;병차치료강도증대,TGF-β1표체증강.③맥충초성파치료가능통과촉진TGF-β1표체이촉진연조직유합.
Objective To investigate the effect of pulsed ultrasound therapy (US) on medial collateral liga-ment healing in rats and it's mechanism. Methods Eighteen 3-month-old male Sprague-Dawley rats with transected medial collateral ligaments (MCLs) were studied. They were randomly divided into a control group, a 0.5 W/cm~2 group and a 1. 0 W/cm~2 group. The control group was not given any treatment. The 0. 5 W/cm~2 group and 1.0 W/cm~2 group were given 10 minutes of pulsed US (duty cycle: on/off = 3 ms/1 ms) daily for 8 days at either 0.5 or 1.0 W/cm~2 intensity. All the rats were sacrificed on the 9th day. After macroscopic examination, their MCLs were harvested and studied using haematoxylin-eosin staining, Van Gieson's staining and immunohistochemical tech-niques in order to detect transforming growth factor beta-1(TGF-β1) and any histological or histochemical changes.Results Macroscopically, the lacerated MCLs had healed with scar tissue formation. Scarring appeared to be greater in the 0.5 W/cm~2 and 1.0 W/cm~2 groups than in the control group. Inflamed cells appeared to be more numerous in the treated groups than in the controls. There were significant differences in collagen fiber extent among all three groups. In the 1.0 W/cm~2 group, the average level of TGF-β1 was significantly up-regulated, and TGF-β1 expres-sion was higher than in the other two groups. Conclusions Pulsed US can improve ligament healing in the short term, however whether long-term treatment with US can yield further improvement is unknown. Pulsed US can in-crease the level of TGF-β1, which will be higher with higher US dosage. Pulsed US may enhance injored ligament re-pair by up-regulating TGF-β1.Objective To investigate the effect of pulsed ultrasound therapy (US) on medial collateral liga-ment healing in rats and it's mechanism. Methods Eighteen 3-month-old male Sprague-Dawley rats with transected medial collateral ligaments (MCLs) were studied. They were randomly divided into a control group, a 0.5 W/cm~2 group and a 1. 0 W/cm~2 group. The control group was not given any treatment. The 0. 5 W/cm~2 group and 1.0 W/cm~2 group were given 10 minutes of pulsed US (duty cycle: on/off = 3 ms/1 ms) daily for 8 days at either 0.5 or 1.0 W/cm~2 intensity. All the rats were sacrificed on the 9th day. After macroscopic examination, their MCLs were harvested and studied using haematoxylin-eosin staining, Van Gieson's staining and immunohistochemical tech-niques in order to detect transforming growth factor beta-1(TGF-β1) and any histological or histochemical changes.Results Macroscopically, the lacerated MCLs had healed with scar tissue formation. Scarring appeared to be greater in the 0.5 W/cm~2 and 1.0 W/cm~2 groups than in the control group. Inflamed cells appeared to be more numerous in the treated groups than in the controls. There were significant differences in collagen fiber extent among all three groups. In the 1.0 W/cm~2 group, the average level of TGF-β1 was significantly up-regulated, and TGF-β1 expres-sion was higher than in the other two groups. Conclusions Pulsed US can improve ligament healing in the short term, however whether long-term treatment with US can yield further improvement is unknown. Pulsed US can in-crease the level of TGF-β1, which will be higher with higher US dosage. Pulsed US may enhance injored ligament re-pair by up-regulating TGF-β1.