CAJ | 학술논문

目的探讨胰腺癌患者外周血DNA中SARP2基因甲基化对胰腺癌的诊断价值.方法收集12例原发性胰腺癌、10例慢性胰腺炎和6例健康志愿者外周静脉血,提取血清游离DNA,经亚硫酸氢盐修饰后,行SARP2基因第一外显子区域的BSP扩增和测序.结果 12例胰腺癌外周血DNA、10例慢性胰腺炎外周血DNA中分别有10例(83%)和4例(40%)检测出明显的SARP2基因甲基化改变.胰腺癌患者外周血中SARP2基因第一外显子区CpG位点甲基化率为16.8%;慢性胰腺炎患者的甲基化率为10.4%,健康志愿者为2.2%.3组间的SARP2 CpG岛甲基化率差别非常显著(P<0.01或P<0.05).结论胰腺癌患者外周血DNA中可以检测到SARP2甲基化改变,SARP2甲基化的检测对胰腺癌的诊断可能有潜在的临床价值.
목적 탐토이선암환자외주혈DNA중SARP2기인갑기화대이선암적진단개치.방법 수집12례원발성이선암、10례만성이선염화6례건강지원자외주정맥혈,제취혈청유리DNA,경아류산경염수식후,행SARP2기인제일외현자구역적BSP확증화측서.결과 12례이선암외주혈DNA、10례만성이선염외주혈DNA중분별유10례(83%)화4례(40%)검측출명현적SARP2기인갑기화개변.이선암환자외주혈중SARP2기인제일외현자구CpG위점갑기화솔위16.8%;만성이선염환자적갑기화솔위10.4%,건강지원자위2.2%.3조간적SARP2 CpG도갑기화솔차별비상현저(P<0.01혹P<0.05).결론 이선암환자외주혈DNA중가이검측도SARP2갑기화개변,SARP2갑기화적검측대이선암적진단가능유잠재적림상개치.
Objective To investigate the diagnostic value of measurement of SARP2 methylation in peripheral blood for detection of pancreatic cancer in human. Methods Peripheral vein blood of 12 patients with primary pancreatic cancers, 10 patients with chronic pancreatitis and 6 health volunteers were collected. Serum free DNA were extracted from blood samples, and were modified with bisulfate, and SARP2 gene extron 1 were amplified through BSP and sequencing of the production. Results There were 12 patients (83 %) with pancreatic cancer and 10 patients (40%) with chronic pancreatitis had obvious methylation in SARP2 gene in peripheral blood. The rate of CpG methylation in SARP2 gene extron 1 of pancreatic cancer, chronic pancreatitis and health volunteers was 16. 8% , 10. 4% and 2. 2% respectively. There was statistically significant difference among the three groups (P<0.01 or P< 0.05). Conclusions Aberrant methylation of SARP2 gene could be detected in peripheral blood in patients with pancreatic cancer, the detection of SARP2 gene methylation may have potential clinical implication for diagnosis of pancreatic cancer.