国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2012年
20期
2948-2950
,共3页
肌成纤维细胞%良性胆管狭窄%丹参%TGF- β 1%α-SMA
肌成纖維細胞%良性膽管狹窄%丹參%TGF- β 1%α-SMA
기성섬유세포%량성담관협착%단삼%TGF- β 1%α-SMA
Myofibroblast (MFB)%Benign biliary stricture%Salvia extraction fluid%Transforming growth factor β 1(TGF- β 1)%α -SMA
目的 探讨丹参萃取液在预防良性胆管狭窄中的作用.方法 通过制作犬胆管损伤修复模型,分离及培养原代肌成纤维细胞,并将MBF细胞分为对照组、实验组和空白对照组,MTT法测定丹参萃取液对MBF细胞增殖率的影响;PCR检测TGF-β 1、α-SMA在MBF中的表达.结果 丹参萃取液干预MBF细胞24 h后,对照组MTT-OD值为(0.3696±0.0304),实验组为(0.1801±0.015 20),MBF细胞的MTT-OD值的细胞百分比均显著低于对照组(P＜0.05).在丹参萃取液的作用下,对照组TGF- β 1为(1.00±0.18),实验组为(0.36±0.04);对照组α-SMA为(1.00±0.230),实验组为(0.41±0.023); MBF细胞的TGF-β 1、α-SMA基因表达显著下降.结论 丹参萃取液作用于MBF细胞使其细胞增殖率下降及凋亡增强,导致TGF-β 1、α-SMA等炎症因子分泌减少,减轻胆道瘢痕的形成,可用于预防良性胆管狭窄.
目的 探討丹參萃取液在預防良性膽管狹窄中的作用.方法 通過製作犬膽管損傷脩複模型,分離及培養原代肌成纖維細胞,併將MBF細胞分為對照組、實驗組和空白對照組,MTT法測定丹參萃取液對MBF細胞增殖率的影響;PCR檢測TGF-β 1、α-SMA在MBF中的錶達.結果 丹參萃取液榦預MBF細胞24 h後,對照組MTT-OD值為(0.3696±0.0304),實驗組為(0.1801±0.015 20),MBF細胞的MTT-OD值的細胞百分比均顯著低于對照組(P＜0.05).在丹參萃取液的作用下,對照組TGF- β 1為(1.00±0.18),實驗組為(0.36±0.04);對照組α-SMA為(1.00±0.230),實驗組為(0.41±0.023); MBF細胞的TGF-β 1、α-SMA基因錶達顯著下降.結論 丹參萃取液作用于MBF細胞使其細胞增殖率下降及凋亡增彊,導緻TGF-β 1、α-SMA等炎癥因子分泌減少,減輕膽道瘢痕的形成,可用于預防良性膽管狹窄.
목적 탐토단삼췌취액재예방량성담관협착중적작용.방법 통과제작견담관손상수복모형,분리급배양원대기성섬유세포,병장MBF세포분위대조조、실험조화공백대조조,MTT법측정단삼췌취액대MBF세포증식솔적영향;PCR검측TGF-β 1、α-SMA재MBF중적표체.결과 단삼췌취액간예MBF세포24 h후,대조조MTT-OD치위(0.3696±0.0304),실험조위(0.1801±0.015 20),MBF세포적MTT-OD치적세포백분비균현저저우대조조(P＜0.05).재단삼췌취액적작용하,대조조TGF- β 1위(1.00±0.18),실험조위(0.36±0.04);대조조α-SMA위(1.00±0.230),실험조위(0.41±0.023); MBF세포적TGF-β 1、α-SMA기인표체현저하강.결론 단삼췌취액작용우MBF세포사기세포증식솔하강급조망증강,도치TGF-β 1、α-SMA등염증인자분비감소,감경담도반흔적형성,가용우예방량성담관협착.
Objective To explore the role of salvia extraction fluid in the benign biliary stricture.Methods A model of trauma of bile duct was established in dogs and then repaired.The myofibroblast (MFB) were extracted by enzymatic digestion and in intro different velocity adherence methods.Through the technique of cell culture in vitro,to establish the model MFB fibrosis induced by Aid,then to add disposal factors. The MFB were divided into 3 groups:control group, experimental group and blank group.After 24 hours of action,proliferation was measured by thiazolyl blue (MTT) assay; the mRNA expression of TGF- β 1 and α -SMA was determined by RT-PCR. Results MTT results showed that cell proliferation rate and apoptosis enhanced in experimental group； gene expression of TGF- β 1 and α -SMA significantly decreased in MFB cells.Conclusions The high expression of TGF- β 1 and α -SMA is closely related to active proliferation of broblasts,extracellular matrix over deposition and scar contracture of bile duct.Salvia extraction fluid could effectively inhibit the proliferation of MBF in a concentration dependent manner,which may play an important role in the regression of cells apoptosis and preventing benign biliary stricture.
