CAJ | 학술논문

目的探讨人乳头瘤病毒(HPV)与皮肤Bowen病发病的相关性.方法 41例皮肤Bowen病患者皮损以及48例健康对照皮肤,采用多对引物,应用巢式PCR进行HPV DNA检测,同时应用半定量PCR进行病毒定量,对HPV DNA阳性标本进一步采用原位杂交方法分析组织内病毒的分布状况.结果 41例皮肤Bowen病患者皮损HPV DNA阳性检出率为12%(5例),其中黏膜型3例(2例HPV16,1例HPV33),病毒定量相当于101～103拷贝,原位杂交显示在多数肿瘤细胞核中有广泛阳性表达,而肿瘤邻近的正常组织无信号表达;皮肤型2例(HPV27和HPV76各1例),原位杂交均无阳性表达.此外,对照组中有1例检出HPV DNA,属于疣状表皮发育不良相关型HPV23,检出率为2.1%,与皮肤Bowen病皮损中HPV检出率比较,差异无统计学意义.2例患者皮肤型病毒定量较低,与正常对照组中检出的1例HPV23相似,均相当于10-2～10-3拷贝.结论某些皮肤Bowen病的发病与黏膜型HPV密切相关.
목적 탐토인유두류병독(HPV)여피부Bowen병발병적상관성.방법 41례피부Bowen병환자피손이급48례건강대조피부,채용다대인물,응용소식PCR진행HPV DNA검측,동시응용반정량PCR진행병독정량,대HPV DNA양성표본진일보채용원위잡교방법분석조직내병독적분포상황.결과 41례피부Bowen병환자피손HPV DNA양성검출솔위12%(5례),기중점막형3례(2례HPV16,1례HPV33),병독정량상당우101～103고패,원위잡교현시재다수종류세포핵중유엄범양성표체,이종류린근적정상조직무신호표체;피부형2례(HPV27화HPV76각1례),원위잡교균무양성표체.차외,대조조중유1례검출HPV DNA,속우우상표피발육불량상관형HPV23,검출솔위2.1%,여피부Bowen병피손중HPV검출솔비교,차이무통계학의의.2례환자피부형병독정량교저,여정상대조조중검출적1례HPV23상사,균상당우10-2～10-3고패.결론 모사피부Bowen병적발병여점막형HPV밀절상관.
Objective To assess the relationship between human papillomavirus (HPV) and extrageni-tal Bowen's disease. Methods Regular PCR with consensus primers for LI region as well as mix primers and nested PCR were performed to detect the DNA of a broad range of cutaneous and mucosal HPV types in tissue samples from lesions of 41 patients with extragenital Bowen's disease and from normal skin of 48 human controls. Semiquantitative PCR and tyramide-based in situ hybridization (ISH) were also conducted to determine the load and localization of HPV DNA in HPV-positive samples. Results HPV DNA was detected in lesions from 5 (12%) of the 41 patients with extragenital Bowen's disease. Of the 5 HPV-positive patients, 3 carried mucosal HPV types (HPV16 in 2 cases, HPV 33 in 1 case) with a viral load of 101 to 103 copies, 2 cutaneous HPV types (HPV27 in 1 case and HPV76 in 1 case). As ISH showed, there was a generalized expression of mucosal HPV DNA in most tumor cell nuclei but not in peritumoral normal tissue, and no expression of cutaneous HPV DNA was observed in lesions. HPV DNA was detected in 1 (2.1%) control tissue sample, which proved to be epidermodysplasia verruciformis-associated HPV23. There was no significant difference in the detection rate of HPV DNA between the patients and controls. The viral load of cutaneous HPV types amounted to 10-2 to 10-3 copies in the 2 patients, which was similar to that of HPV 23 in the normal control. Conclusion Mucosal HPV types may be closely associated with the development of extragenital Bowen's disease.