CAJ | 학술논문

目的初步探讨斑蝥酸钠对人胃癌裸鼠皮下移植瘤生长的作用及其可能的作用机制.方法建立人胃癌细胞系BGC823裸鼠皮下移植瘤模型,观察斑蝥酸钠对移植瘤生长的影响,同时应用免疫组织化学和TUNEL法分别检测斑蝥酸钠作用前后移植瘤细胞的增殖活性和细胞凋亡情况.结果不同浓度的斑蝥酸钠均能明显抑制裸鼠皮下移植瘤的生长(P＜0.01);斑蝥酸钠作用后肿瘤细胞的增殖活性明显低于作用前(P＜0.01);斑蝥酸钠作用后肿瘤细胞的凋亡指数显著高于对照组(P＜0.01).结论斑蝥酸钠对人胃癌棵鼠皮下移植瘤的生长具有明显的抑制作用,其作用机制可能是通过抑制肿瘤细胞增殖或诱导肿瘤细胞凋亡而实现的.
목적 초보탐토반모산납대인위암라서피하이식류생장적작용급기가능적작용궤제.방법 건립인위암세포계BGC823라서피하이식류모형,관찰반모산납대이식류생장적영향,동시응용면역조직화학화TUNEL법분별검측반모산납작용전후이식류세포적증식활성화세포조망정황.결과 불동농도적반모산납균능명현억제라서피하이식류적생장(P＜0.01);반모산납작용후종류세포적증식활성명현저우작용전(P＜0.01);반모산납작용후종류세포적조망지수현저고우대조조(P＜0.01).결론 반모산납대인위암과서피하이식류적생장구유명현적억제작용,기작용궤제가능시통과억제종류세포증식혹유도종류세포조망이실현적.
Objective To study the effect of sodium cantharidinate on the growth of human gastric cancer grafted onto nude mice. Methods Nude mice xenograft models of human gastric cancer were established.BGC823was injected peritoneal and the mice were weighed. The proliferating and apoptosis rates of xenografta was evaluated by TUNEL assay and immunohistochemical staining respectively. Results The xenografts were obviously inhibited with various dosage of sodium cantharidinate(P＜0.01 ), the proliferating rate of turnout cells after using sodium cantharidinate was lower than that before using sodium cantharidinate(P＜0.01 ), but apoptosis rate of tumour ceils after using sodium cantharidinate was higher than that before using sodium cantharidinate(P＜0.01 ). Conclusion Sodium cantharidinate can inhibit gastric cancer growth by inhibiting tumour cell proliferating or inducing cell apoptosls.