CAJ | 학술논문

目的寻找可能存在的抗丙型肝炎病毒(HCV)感染的中和抗体。方法构建两个分别含HCV E1和E2抗原基因的真核表达载体, 用瞬时表达法检测其在哺乳动物细胞中的表达。将构建的载体连同IL-2基因一起经皮下给BalB/c小鼠进行注射，然后用ELISA法检测特异性抗体产生情况。最后通过研究抗体和病毒间的相互作用分析基因免疫诱导产生的抗血清在体外与HCV的相互作用情况。结果经过基因免疫的小鼠分别产生了E-1和E2抗体。其中，用含E2基因的质粒载体免疫的小鼠所产生的E2抗血清不仅可以免疫沉淀来源血清中的HCV病毒颗粒，而且可以和与来源株非相关的HCV病毒颗粒相互作用。结论我们的研究表明基因免疫诱导小鼠产生的E2抗体可以和HCV病毒颗粒发生交叉反应，这使我们看到了诱导产生具有广泛针对性的E2抗体的希望。
목적심조가능존재적항병형간염병독(HCV)감염적중화항체。 방법구건량개분별함HCV E1화E2항원기인적진핵표체재체, 용순시표체법검측기재포유동물세포중적표체。장구건적재체련동IL-2기인일기경피하급BalB/c소서진행주사，연후용ELISA법검측특이성항체산생정황。최후통과연구항체화병독간적상호작용분석기인면역유도산생적항혈청재체외여HCV적상호작용정황。 결과경과기인면역적소서분별산생료E-1화E2항체。기중，용함E2기인적질립재체면역적소서소산생적E2항혈청불부가이면역침정래원혈청중적HCV병독과립，이차가이화여래원주비상관적HCV병독과립상호작용。 결론아문적연구표명기인면역유도소서산생적E2항체가이화HCV병독과립발생교차반응，저사아문간도료유도산생구유엄범침대성적E2항체적희망。
Objective　To find neutralizing antibody candidates against hepatitis C virus (HCV) infection. Methods　We constructed two eukaryotic expression vectors which contained the E1 and E2 gene of HCV, and detected their expression in mammalian cells with transient expression. BALB/c mice were given subculaneous injections of constructed vectors combined with the IL-2 gene intraepidermally and evaluated for induced humoral immune responses by enzyme-linked immunosorbent assay (ELISA). We used an antibody-virus interaction assay to analyze the interaction of the antisera and HCV viral particles in vitro. Results　Anti E1 and anti-E2 antisera were obtained from immunized mice. The serum of mice immunized with the E2 gene immunoprecipitated the HCV isolate in source serum and reacted with the isolates unrelated to the original one. Conclusions　Anti-E2 antibody in induced mice can cross-reactively capture HCV particles, highlighting the possibility of generating broadly reactive anti-E2 antibodies.