CAJ | 학술논문

目的研究云南省人类免疫缺陷病毒-1(HIV-1)亚型的地理及人群分布.方法采集云南省2001-2006年1319名HIV-1感染者血液标本,分离血清后提取病毒RNA,用巢式PCR方法对gag基因片段进行扩增.扩增产物经基因序列测定,测序结果用进化树分析方法进行亚型分析.结果成功获得了644名HIV感染者的基因序列,发现云南存在7种HIV-1亚型的流行,包括CRF08_BC、CRF07_BC、CRF07/08_BC,CRF01_AE、C、B'及URFB/C.C/CRF07_BC/CRF08_BC在各地均存在,而CRF01_AE主要存在于与缅甸接壤的德宏、保山、西双版纳和普洱.此外,还发现在感染C/CRF07_BC/CRF08_BC的人群中,61.6%(270/438)为注射吸毒感染,而感染CRF01_AE的人群中,注射吸毒传播仅占8.5%(15/177).结论云南省存在7种HIV-1亚型流行,亚型分布与传播途径相关,并存在地域分布上的差异.
목적 연구운남성인류면역결함병독-1(HIV-1)아형적지리급인군분포.방법 채집운남성2001-2006년1319명HIV-1감염자혈액표본,분리혈청후제취병독RNA,용소식PCR방법대gag기인편단진행확증.확증산물경기인서렬측정,측서결과용진화수분석방법진행아형분석.결과 성공획득료644명HIV감염자적기인서렬,발현운남존재7충HIV-1아형적류행,포괄CRF08_BC、CRF07_BC、CRF07/08_BC,CRF01_AE、C、B'급URFB/C.C/CRF07_BC/CRF08_BC재각지균존재,이CRF01_AE주요존재우여면전접양적덕굉、보산、서쌍판납화보이.차외,환발현재감염C/CRF07_BC/CRF08_BC적인군중,61.6%(270/438)위주사흡독감염,이감염CRF01_AE적인군중,주사흡독전파부점8.5%(15/177).결론 운남성존재7충HIV-1아형류행,아형분포여전파도경상관,병존재지역분포상적차이.
Objective To analyze the geographical distribution and risk factors of HIV-1 subtypes in Yunnan orovince.Methods Blood samples from 1319 HIV positives were collected in Yunnan Province from 2001 to 2006.The nested polymerase chain reaction was used to amplify the gag(p24)-protease fragments from RNA extracted from plasma or sera.The sequences were used for subtype determination by phylogenetic tree analysis.Results Among 1319 samples studied,the subtypes has been successfully obtained from 644 samples that were constituted of seven subtypes:CRF08_BC,CRF07_BC,CRF07/08_BC,CRFD1_AE,C,B'and URFB/C.C/CRF07_BC/CRF08_BC were distributed in the whole province,but CRF01_AE were mainly distributed in the boarding areas with Myanmar such as Dehong,Baoshan,Xishuangbanna and Puer.Moreover,injecting drugs users accounted for 61.6%(270/438)among C/CRF07_BC/CRF08_BC infections,while only 8.5%(15/177)among CRF01_AE infections.Conclusion Our data indicated that at least seven subtypes were idenfified in Yunnan province,the relationship between subtypes and transmission routes were analyzed,and the geographic difference of subtypes was also observed.