CAJ | 학술논문

目的探讨核因子NF-κ B和转移抑制基因nm23-H1在肝细胞癌(HCC)组织的表达情况及与临床病理特征的关系.方法采用免疫组织化学方法检测41例HCC中NF-κB和nm23-H1的表达情况,以相应癌旁肝组织、肝硬化组织(10例)、肝血管瘤旁正常肝组织(11例)作对照,并分析其与病理特征的关系.结果 NF-κB蛋白在41例HCC组织中阳性28例,表达率为68.3%;41例相应癌旁肝组织中有5例表达,阳性率12.2%,10例肝硬变组织中1例表达,11例正常肝组织未见表达,HCC与非癌组织中NF-κB蛋白的表达差异有统计学意义(χ2=41.1,P＜0.01);NF-κB的表达与HCC的临床病理特点无显著相关性.nm23-H1蛋白在41例HCC组织中14例为阳性染色,27例为阴性染色.nm23-H1阴性表达与肝被膜浸润和门静脉浸润显著相关(χ2=4.16,χ2=4.19,P均＜0.05).结论 NF-κB可能参与了HCC的发生、发展,可望作为基因治疗的靶点.nm23-H1在HCC中的阴性表达与HCC的恶性生物学特征相关,可能表明肿瘤的预后不良.
목적 탐토핵인자NF-κ B화전이억제기인nm23-H1재간세포암(HCC)조직적표체정황급여림상병리특정적관계.방법 채용면역조직화학방법검측41례HCC중NF-κB화nm23-H1적표체정황,이상응암방간조직、간경화조직(10례)、간혈관류방정상간조직(11례)작대조,병분석기여병리특정적관계.결과 NF-κB단백재41례HCC조직중양성28례,표체솔위68.3%;41례상응암방간조직중유5례표체,양성솔12.2%,10례간경변조직중1례표체,11례정상간조직미견표체,HCC여비암조직중NF-κB단백적표체차이유통계학의의(χ2=41.1,P＜0.01);NF-κB적표체여HCC적림상병리특점무현저상관성.nm23-H1단백재41례HCC조직중14례위양성염색,27례위음성염색.nm23-H1음성표체여간피막침윤화문정맥침윤현저상관(χ2=4.16,χ2=4.19,P균＜0.05).결론 NF-κB가능삼여료HCC적발생、발전,가망작위기인치료적파점.nm23-H1재HCC중적음성표체여HCC적악성생물학특정상관,가능표명종류적예후불량.
Objective To study the expression of nuclear transcription faetor-KB(NF-κB)and nm23-H1 protein in hepatocellular carcinoma(HCC)and its relationship with clinical pathologic features.Methods The expression of NF-κB and nm23-H1 in 41 cases of HCC were examined by immunohistochemical technique.The corresponding cancerous adjacent tissue or cirrhosis tissue(10 cases)and normal liver tissue (11 cases)collected from specimens of benign liver hemangioma were served as a control group.Results NF-κB protein was detected in 68.3%(28/41)cases of HCC tissues compared with 12.2%(5/41)in noncancerous adjacent tissues,1/10 cases in liver cirrhosis,0/11 cases in normal liver tissues(P＜0.01).NF-κB expression has no correlation with clinical pathological feature of HCC patients fourteen cases of 41 HCC tissues were found nm23-H1 protein positive staining,compared with 27 cases negative staining;negative expression of nm23-H1 protein correlates closely with portal vein invasion and serosal infiltration(χ2=4.16χ2=4.19,P＜0.05),and its negative expression correlates well with survivin expression(χ2=20.25,P＜0.01).Conclusion High expression of NF-κB protein in HCC tissues suggests that it probably played an important role in the origin and development of HCC,and it may be as targets for gene therapy.Negative expression of nm23-H1 protein correlates with malignant biological feature of HCC,and probably suggests poor prognosis.