CAJ | 학술논문

目的研究低频电刺激对急性局灶性脑梗死大鼠运动功能和梗死边缘区胶质纤维酸性蛋白(GFAP)表达的影响,探讨低频电刺激治疗促进脑梗死后运动功能恢复的机制.方法 54只成年雄性SD大鼠,随机分为低频电刺激组、模型对照组及假手术组,每组18只,每组动物再分为治疗3,7和14 d 3个时间点,每个时间点6只.参照Longa等的线栓法制成左侧大脑中动脉闭塞(MCAO)模型.低频电刺激组于造模术后3 d开始进行低频电刺激治疗,模型对照组应用相同的低频电刺激仪治疗,但不予以电流刺激,假手术组不予任何治疗.分别在治疗前和治疗后各时间点给予平衡木行走测评、转棒上行走测评和网屏试验等功能评定;以免疫组织化学技术观察梗死边缘区胶质纤维酸性蛋白(GFAP)的表达水平变化.结果低频电刺激组大鼠运动功能较模型对照组明显改善(P<0.05).低频电刺激组缺血性半影区的GFAP阳性率较模型对照组高(P<0.05).结论低频电刺激能促进脑梗死大鼠运动功能恢复,其中一个重要机制可能是低频电刺激能增强GFAP的表达,促进缺血后脑的可塑性变化,构成了脑梗死后功能恢复的物质基础.
목적 연구저빈전자격대급성국조성뇌경사대서운동공능화경사변연구효질섬유산성단백(GFAP)표체적영향,탐토저빈전자격치료촉진뇌경사후운동공능회복적궤제.방법 54지성년웅성SD대서,수궤분위저빈전자격조、모형대조조급가수술조,매조18지,매조동물재분위치료3,7화14 d 3개시간점,매개시간점6지.삼조Longa등적선전법제성좌측대뇌중동맥폐새(MCAO)모형.저빈전자격조우조모술후3 d개시진행저빈전자격치료,모형대조조응용상동적저빈전자격의치료,단불여이전류자격,가수술조불여임하치료.분별재치료전화치료후각시간점급여평형목행주측평、전봉상행주측평화망병시험등공능평정;이면역조직화학기술관찰경사변연구효질섬유산성단백(GFAP)적표체수평변화.결과 저빈전자격조대서운동공능교모형대조조명현개선(P<0.05).저빈전자격조결혈성반영구적GFAP양성솔교모형대조조고(P<0.05).결론 저빈전자격능촉진뇌경사대서운동공능회복,기중일개중요궤제가능시저빈전자격능증강GFAP적표체,촉진결혈후뇌적가소성변화,구성료뇌경사후공능회복적물질기출.
Objective To study the effects of low-frequency electrical stimulation(LFES)on motor function and the expression of glia fibrillary acidic protein(GFAP)around cerebral infarction sites in rats.Methods Fifty-four male adult Sprague-Dawley rats were randomly divided into a LFES group,a placebo group and a sham operation group(18/group).All groups were randomly divided into 3 treatment groups.A rat model of middle cerebral artery occlusion(MCAO)was established using intraluminal filament occlusion.Treatment was carried out 3 d after the operation.Rats in the LFES treatment groups were stimulated with LFES for 3,7 or 14 days (10 min/d);the placebo groups were treated in the same way without electric stimulation;the sham operation subgroups didn't receive any therapy.Scores on a beam-walking test,a rotating pole test and a screen test were assessed at each time point mentioned above.Expression of GFAP was also assessed using immunohistochemcal techniques.Results The paralysed limbs recovered motor function better in the LFES groups than in the control groups.GFAP-positive cells were more numerous at the margins of the infarction area in the treated groups than in the control groups.Conclusions LFES might increase the expression of GFAP,which might be an important mechanism in improving brain plasticity after cerebral ischemia,aiding the recovery of the central nervous system and rebuilding its functioning.