CAJ | 학술논문

目的应用基因芯片技术从基因水平初步了解烧伤后增生性瘢痕形成的机制。方法按一步法抽提3例烧伤患者的增生性瘢痕及其自身正常皮肤组织的总RNA，纯化 mRNA；将4 096种人类基因PCR产物用 Cartesian Pixsys 7500 点样仪微矩阵列点样于化学涂层的载玻片上，制成基因芯片；将等量的增生性瘢痕和患者自身正常皮肤组织mRNA 分别逆转录合成荧光标记的cDNA混合物探针，与上述基因芯片杂交。经严格洗片后，用SanArray 3000 扫描仪扫描芯片荧光信号图像，计算机分析后比较两种组织中差异表达的基因。结果在4 096种基因中，患者的增生性瘢痕及其自身正常皮肤组织间存在差异表达基因。在所检测的3例临床标本中，共有差异表达基因128条。结论包括细胞凋亡基因、免疫相关基因、细胞骨架和运动基因、原癌基因和抑癌基因、细胞信号和传递蛋白基因等多种基因参与了增生性瘢痕的发生。
목적응용기인심편기술종기인수평초보료해소상후증생성반흔형성적궤제。방법안일보법추제3례소상환자적증생성반흔급기자신정상피부조직적총RNA，순화 mRNA；장4 096충인류기인PCR산물용 Cartesian Pixsys 7500 점양의미구진렬점양우화학도층적재파편상，제성기인심편；장등량적증생성반흔화환자자신정상피부조직mRNA 분별역전록합성형광표기적cDNA혼합물탐침，여상술기인심편잡교。경엄격세편후，용SanArray 3000 소묘의소묘심편형광신호도상，계산궤분석후비교량충조직중차이표체적기인。결과재4 096충기인중，환자적증생성반흔급기자신정상피부조직간존재차이표체기인。재소검측적3례림상표본중，공유차이표체기인128조。결론포괄세포조망기인、면역상관기인、세포골가화운동기인、원암기인화억암기인、세포신호화전체단백기인등다충기인삼여료증생성반흔적발생。
Objective　 To screen the differently expressed genes between postburn hypertrophic scar and normal skin tissue using cDNA microarray. 　Methods　The total RNAs from 3 cases were isolated from the tissues and purified to mRNAs by oligotex. The PCR products of 4 096 genes were spotted onto a chemical substance-coated glass plate in array using Cartesian Pixsys 7 500. DNA was fixed onto the glass plate after series of treatments. Both mRNAs from the postburn hypertrophic scar and normal skin tissue were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray scanned for the fluorescent signals and showed differences between 2 tissues.　 Results　Among 4 096 target genes, the expression level of 128 genes differed in 3 cases between the postburn hypertrophic scar and normal skin tissue. Conclusions　Genes including the apoptotic genes, immune related genes, cellular signal and transferring genes take part in the development of postburn hypertrophic scar.