CAJ | 학술논문

目的探讨程序化细胞死亡因子5(PDCD5)在支气管哮喘患者血清中的表达及其意义.方法将2011年6-12月北京大学第三医院呼吸科门诊及病房诊治的40例成人支气管哮喘患者作为哮喘组(其中急性发作期12例,慢性持续期14例,临床缓解期14例),15名同期在体检中心进行健康体检者作为对照组.收集并检测两组受试者外周血中性粒细胞、嗜酸粒细胞百分数,酶联免疫吸附法测定血清PDCD5水平,填写哮喘控制测试(ACT)问卷,使用肺功能仪测定肺功能相关指标；对检测结果进行统计学分析.结果哮喘患者外周血中性粒细胞及嗜酸粒细胞百分数与对照组比较,差异均无统计学意义(均P＞0.05),哮喘组血清PDCD5表达量显著高于对照组[(47.7±29.6)比(19.3±9.8)μg/L,P＜0.05],急性发作期、慢性持续期患者血清PDCD5显著高于临床缓解期[(55.2±24.5)、(68.5±22.1)比(16.0±7.9)μg/L,均P＜0.05 ],哮喘患者血清PDCD5表达量与第1秒用力呼气量(FEV1)占预计值的百分比(FEV1％)、FEV1与用力肺活量的比值(FEV1/FVC)及ACT问卷评分呈负相关(r=-0.539～-0.798,均P＜0.05).结论 PDCD5参与了哮喘的气道炎症过程,其表达异常可能与哮喘末控制有关,可能成为临床上协助评估哮喘控制情况的指标或治疗的靶点.
목적 탐토정서화세포사망인자5(PDCD5)재지기관효천환자혈청중적표체급기의의.방법 장2011년6-12월북경대학제삼의원호흡과문진급병방진치적40례성인지기관효천환자작위효천조(기중급성발작기12례,만성지속기14례,림상완해기14례),15명동기재체검중심진행건강체검자작위대조조.수집병검측량조수시자외주혈중성립세포、기산립세포백분수,매련면역흡부법측정혈청PDCD5수평,전사효천공제측시(ACT)문권,사용폐공능의측정폐공능상관지표；대검측결과진행통계학분석.결과 효천환자외주혈중성립세포급기산립세포백분수여대조조비교,차이균무통계학의의(균P＞0.05),효천조혈청PDCD5표체량현저고우대조조[(47.7±29.6)비(19.3±9.8)μg/L,P＜0.05],급성발작기、만성지속기환자혈청PDCD5현저고우림상완해기[(55.2±24.5)、(68.5±22.1)비(16.0±7.9)μg/L,균P＜0.05 ],효천환자혈청PDCD5표체량여제1초용력호기량(FEV1)점예계치적백분비(FEV1％)、FEV1여용력폐활량적비치(FEV1/FVC)급ACT문권평분정부상관(r=-0.539～-0.798,균P＜0.05).결론 PDCD5삼여료효천적기도염증과정,기표체이상가능여효천말공제유관,가능성위림상상협조평고효천공제정황적지표혹치료적파점.
Objective To explore the expression and significance of serum programmed cell death 5 (PDCD5) in patients with bronchial asthma.Methods From June to December 2011,a total of 40 adults with bronchial asthma treated in Peking University Third Hospital were enrolled.Among them,the categories were acute phase (n=12),chronic phase (n=14) and remission phase (n=14).Fifteen healthy adults were selected into the control group.The percentages of peripheral blood neutrophils and eosionphils were collected and detected for each patient.Serum PDCD5 was detected with enzyme-linked immunosorbent assay (ELISA) and asthma control test (ACT) questionnaire filled in.The relevant pulmonary functional indicators were analyzed with a pulmonary spirometer. Two-independent sample t-test and Pearson's correlation analysis were used for statistical analysis.Results No significant difference was found between two groups with regards to the percentages of peripheral blood eosinophils and neutrophils ( all P＞0.05 ).Serum PDCD5 was significantly higher in the patient group ( ( 47.7±29.6) vs ( 19.3±9.8 ) μg/L,P ＜0.05 ).Patients of chronic and acute phases showed a significant higher expression in PDCD5 than the remission phase ((55.2±24.5) & (68.5±22.1) vs (16.0±7.9) μg/L,both P ＜0.05).Serum PDCD5 of asthmatics showed a negative correlation with FEV1％,FEV1/FVC ratio and ACT scores (r =-0.539 to -0.798,all P＜0.05 ).Conclusions PDCD5 participates in the inflammatory process of asthmatic airway.Its abnormal expression may be associated with the uncontrolled state of asthmatics.It may serve as an indicator of assessing the levels of asthma control or a target for the treatment of asthma.