CAJ | 학술논문

目的观察单核细胞趋化蛋白-1(MCP-1)、小干扰RNA(siRNA)、转染兔颈动脉粥样硬化(AS)模型对局部MCP-1表达及AS早期形成的影响.方法建立28只雄性兔颈动脉AS模型,将MCP-1 siRNA真核表达质粒通过局部定位转染的方法至病变血管,病理形态学观察血管壁中泡沫细胞浸润及内膜增厚,免疫组织化学、逆转录.聚合酶链反应(RT-PCR)、Western blot检测局部MCP-1表达.结果模型对照组、空质粒组、RNA干扰(RNAi)组镜下内膜/中膜厚度(I:M)分别为5.55、5.27、1.46.免疫组织化学染色显示RNAi组着色较浅.RT-PeR及Western blot结果显示:RNAi组与模型对照组、空质粒组比较,局部MCP-1表达降低.结论 MCP-1 siRNA转染抑制了局部MCP-1表达,延缓了AS的发展,减轻了病变程度.
목적 관찰단핵세포추화단백-1(MCP-1)、소간우RNA(siRNA)、전염토경동맥죽양경화(AS)모형대국부MCP-1표체급AS조기형성적영향.방법 건립28지웅성토경동맥AS모형,장MCP-1 siRNA진핵표체질립통과국부정위전염적방법지병변혈관,병리형태학관찰혈관벽중포말세포침윤급내막증후,면역조직화학、역전록.취합매련반응(RT-PCR)、Western blot검측국부MCP-1표체.결과 모형대조조、공질립조、RNA간우(RNAi)조경하내막/중막후도(I:M)분별위5.55、5.27、1.46.면역조직화학염색현시RNAi조착색교천.RT-PeR급Western blot결과현시:RNAi조여모형대조조、공질립조비교,국부MCP-1표체강저.결론 MCP-1 siRNA전염억제료국부MCP-1표체,연완료AS적발전,감경료병변정도.
Objective To identify the effects of in vivo transfection of monocyte chemotactic pro- tein-1(MCP-1)small interference RNA (siRNA) to atherosclemsis model on local expression of MCP-1 and the development of atheroscleresis. Methods Carotid atherosclerosis model was created on 28 New Zealand white rabbits, siRNA expressing vector was transfected to the blood vessels. The carotid artery was processed for morphologic evaluation. The local expression of MCP-1 was detected by immunohistochemis-try,RT-PCR and Western blot. Results The intima:media in thickness in RNA interference,control and blank plasmid groups was 5.55,5.27, and 1.46 respectively. Immunohistochemistry showed, as compared with control and blank plasmid groups, the positie staining of MCP-1 was decreased in RNA interference group. RT-PCT and Western blot revealed that MCP-1 expression was reduced in RNA interference group as compared with model and blank plasmid groups. Conclusion The expression of MCP-1 was successful- ly inhibited by transfecting MCP-1 siRNA expression plasmid to the carotid artery, and the progression of atherosclemsis was limited by RNA interference-mediated silencing of MCP-1 expression.