CAJ | 학술논문

目的探讨针刺督脉经穴和夹脊穴对脊髓损伤大鼠神经功能恢复和内源性神经干细胞增殖的影响.方法 48只6周龄SD大鼠以改良Allen wD法制作成脊髓损伤模型,并按随机数字表法分为A组和B组,每组义分为14d、30d、60d和90d4个时间点.A组每天针刺督脉经穴和夹脊穴1次,B组不针刺.各组在相应时间点取材前连续3 d腹腔注射BrdU标记增殖的内源性神经干细胞.各组分别在术后1 d、14 d、30 d、60 d和90d用Tarlov评分和IP评分评价双后肢功能,术后14 d、30 d、60 d和90 d评分后取材进行抗BrdU免疫组化染色.观察各切片BrdU阳性细胞数目及分布情况.结果双后肢功能评分显示术后14 d、30 d、60 d和90 d A组双下肢功能改善与B组比较差异有统汁学意义(P<0.05),功能改善与治疗时间延长平行.免疫组化染色显示术后14 d、30 d、60 d和90 d A组BrdU阳性细胞数和B组比较差异有统计学意义(P<0.05).结论针刺督脉经穴和夹脊穴能促进脊髓损伤大鼠双下肢功能恢复和原位诱导脊髓内源性神经干细胞增殖.
목적 탐토침자독맥경혈화협척혈대척수손상대서신경공능회복화내원성신경간세포증식적영향.방법 48지6주령SD대서이개량Allen wD법제작성척수손상모형,병안수궤수자표법분위A조화B조,매조의분위14d、30d、60d화90d4개시간점.A조매천침자독맥경혈화협척혈1차,B조불침자.각조재상응시간점취재전련속3 d복강주사BrdU표기증식적내원성신경간세포.각조분별재술후1 d、14 d、30 d、60 d화90d용Tarlov평분화IP평분평개쌍후지공능,술후14 d、30 d、60 d화90 d평분후취재진행항BrdU면역조화염색.관찰각절편BrdU양성세포수목급분포정황.결과 쌍후지공능평분현시술후14 d、30 d、60 d화90 d A조쌍하지공능개선여B조비교차이유통즙학의의(P<0.05),공능개선여치료시간연장평행.면역조화염색현시술후14 d、30 d、60 d화90 d A조BrdU양성세포수화B조비교차이유통계학의의(P<0.05).결론 침자독맥경혈화협척혈능촉진척수손상대서쌍하지공능회복화원위유도척수내원성신경간세포증식.
Objective To observe the neurological recovery and endogenous neural stem cell proliferation in rats with spinal cord injury after acupuncture on Governing Vessel and Jiaji (Ex-B2) acupoints. Methods Forty-eight adult SD rats with severe spinal cord injury at the T10 level induced with the method of Allen were randomly divided into two groups (A and B), and each was further randomized into 4 subgroups. In group A, acupuncture was performed once daily on the Governing Vessel and Jiaji (Ex-B2) points, which was not performed in group B. Bromodeoxyuridine (BrdU) was administered intraperitoneally twice daily 3 days prior to heart perfusion for labeling the proliferating endogenous neural stem cells. Open field and inclined plane tests were performed to test the function of the rear limbs of the rats at 1, 14, 30, 60, 90 days after the surgery. The 4 subgroups of rats were sacrificed on days 14, 30, 60 and 90 after the surgery, and the number of BrdU-positive cells and their distribution in the spinal cord was observed in each subgroup using immunohistochemistry with anti-BrdU. Results The rats in group A showed significantly improved neurological function than those in group B (P<0.05). The number of BrdU-positive cells in group A was significantly different from that in group B at each of the time points (P<0.05). Conclusion Acupuncture on the Governing Vessel and Jiaji (Ex-B2) acupoints can promote the functional recovery of the rear limbs and induce the proliferation of endogenous neural stem ceils in situ in rats following spinal cord injury.