CAJ | 학술논문

凉山苦荞茶已形成10亿元的规模化生产产业，而且生产发展不断的加快。因苦荞中的总黄酮在医学临床上具有抗氧化，抗癌，抗炎，降血压等作用。在检测中，由于苦荞含有一定的油脂，在甲醇提取后，在显色时加入AlCl3，乙酸钾水溶液，随着甲醇浓度降低，油脂类物质在甲醇溶液中溶解度降低，而使溶液混浊，影响吸光度的测定。将NY／T1295—2007中的提取总黄酮的方法改进为：称取样品后，加5ml乙醚，将苦荞制品中的油脂类物质溶解，分离、挥发除去乙醚后，再用甲醇提取黄酮，直接显色，显色液清亮透明，省去离心分离，重复洗涤，减少影响因素，便于后期操作。方法简便，确保总黄酮在甲醇和水（7＋3）相中测定，有效排除油脂干扰，检测效果好。
량산고교다이형성10억원적규모화생산산업，이차생산발전불단적가쾌。인고교중적총황동재의학림상상구유항양화，항암，항염，강혈압등작용。재검측중，유우고교함유일정적유지，재갑순제취후，재현색시가입AlCl3，을산갑수용액，수착갑순농도강저，유지류물질재갑순용액중용해도강저，이사용액혼탁，영향흡광도적측정。장NY／T1295—2007중적제취총황동적방법개진위：칭취양품후，가5ml을미，장고교제품중적유지류물질용해，분리、휘발제거을미후，재용갑순제취황동，직접현색，현색액청량투명，성거리심분리，중복세조，감소영향인소，편우후기조작。방법간편，학보총황동재갑순화수（7＋3）상중측정，유효배제유지간우，검측효과호。
Liangshan bitter buckwheat tea has formed 1000000000 of the scale of industrial production, development and production continued to accelerate. Because in tartary buckwheat flavonoids in clinical medicine has antioxidant, anti- cancer, anti-inflammatory, lower blood pressure and other functions. In the detection, the buckwheat contains certain fats, in methanol extraction in color, adding A1C13, potassium acetate aqueous solution, with methanol concentration decreased, fats in methanol solution solubility is reduced, and the turbidity of the solution, the influence of absorbance. NY / T1295-2007 in the extraction of total flavonoids method improvement for, said to take samples, plus 5ml ether, the buck- wheat products of oil substances dissolved, after the separation of volatile to ether, then the methanol extraction of flavonoids, direct color, a color liquid is clear and transparent, eliminating centrifugal separation, repeated washing, reduce the impact factors, which is convenient for later operations. The method is simple, to ensure that the total flavone in methanol and water （ 7＋3 ） phase determination, effectively remove grease disturbance, detection effect is better.