CAJ | 학술논문

建立一种反相高效液相色谱法测定黄酒中氨基甲酸乙酯的方法。样品经固相萃取柱净化后衍生，采用反相C18色谱柱XB—Cfsf4．6mm×250mm,5μm），以乙腈-0．02moL／L乙酸纳水溶液为流动相进行梯度洗脱，流速为lmL／min；设定荧光检测器中激发波长为233nm，发射波长为600nm，对黄酒中的氨基甲酸乙酯进行检测。结果表明，在50．0-250．0μg／L添加量范围内，平均回收率为92．9％，相对标准偏差为4．5％（n=6）。方法的检出限为10μg／L，线性范围为100～1500μg／L（R=0．9995），测定结果与标准气相色谱一质谱法基本相同。所建立的方法可以作为黄酒中氨基甲酸乙酯的检测方法。
건립일충반상고효액상색보법측정황주중안기갑산을지적방법。양품경고상췌취주정화후연생，채용반상C18색보주XB—Cfsf4．6mm×250mm,5μm），이을정-0．02moL／L을산납수용액위류동상진행제도세탈，류속위lmL／min；설정형광검측기중격발파장위233nm，발사파장위600nm，대황주중적안기갑산을지진행검측。결과표명，재50．0-250．0μg／L첨가량범위내，평균회수솔위92．9％，상대표준편차위4．5％（n=6）。방법적검출한위10μg／L，선성범위위100～1500μg／L（R=0．9995），측정결과여표준기상색보일질보법기본상동。소건립적방법가이작위황주중안기갑산을지적검측방법。
A reverse phase HPLC method for the determination of ethyl carbamate in yellow rice wine had been developed. Wine samples were firstly pretreated by solid-phase extraction,ethyl carbamate was separated on a XB- CL8 （4.6mm×250 mm,5 μm） by using gradient elution （acetonitrile and 0.02 moL/L sodium acetate as the mobile phase） at a flow rate of lmL/min and detected by a fluorescence detector （kex=233 nm, hem=600 nm）. At the spike levels of 50.0,100.0 and 250.0μg/L, the average recovery for ethyl carbamate was 92.9% with RSD as 4.5 % （n = 6）, the detection limit was 10 μg/L, and the linear range was from 100-500μg/L（R=0.9995.The determination results by this method were almost the same as those by GCMS. Therefore, this method could be use to determine ethyl carbamate in yellow rice wine.