中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2011年
7期
40-42
,共3页
麻丽丹%王殿夫%于晓婕%吴斌
痳麗丹%王殿伕%于曉婕%吳斌
마려단%왕전부%우효첩%오빈
河豚鱼%皮下充血病%河弧菌%生物学特性%实时PCR
河豚魚%皮下充血病%河弧菌%生物學特性%實時PCR
하돈어%피하충혈병%하호균%생물학특성%실시PCR
swellfish%dermahemia of abdominal regiomV.fluvialis%biological characteristic%real-time PCR
从鳍基部、躯干部以及腹面出现斑点状出血的患病河豚鱼病灶处分离出11株优势茵（M1-M11），以体壁肌肉注射方式进行感染实验，证实菌株M2、M3-M6和M10为养殖河豚鱼鳍基部、躯干部以及腹面出现斑点状出血的病原菌，经形态学观察、生理生化特性分析和实时PCR检测，鉴定结果均为河弧菌。对15种药物的敏感性试验证实该菌株对氯霉素、环丙沙星和诺氟沙星较为敏感。
從鰭基部、軀榦部以及腹麵齣現斑點狀齣血的患病河豚魚病竈處分離齣11株優勢茵（M1-M11），以體壁肌肉註射方式進行感染實驗，證實菌株M2、M3-M6和M10為養殖河豚魚鰭基部、軀榦部以及腹麵齣現斑點狀齣血的病原菌，經形態學觀察、生理生化特性分析和實時PCR檢測，鑒定結果均為河弧菌。對15種藥物的敏感性試驗證實該菌株對氯黴素、環丙沙星和諾氟沙星較為敏感。
종기기부、구간부이급복면출현반점상출혈적환병하돈어병조처분리출11주우세인（M1-M11），이체벽기육주사방식진행감염실험，증실균주M2、M3-M6화M10위양식하돈어기기부、구간부이급복면출현반점상출혈적병원균，경형태학관찰、생리생화특성분석화실시PCR검측，감정결과균위하호균。대15충약물적민감성시험증실해균주대록매소、배병사성화낙불사성교위민감。
Eleven bacterial strains named M1-Mll were isolated from the cultured swellfish with dermahemia in abdominal. pinna and truncal region.The strains M2,M3-M6 and M10 were proved to be pathogen by muscle injection.By meens of morphological observation, physiological and biochemical characteristic analysis and PCR detection, They were identified as Vibrio fluvialis.The results of sensitivity test with 15 antibacterial agents showed that they were sensitive to Chloramphenicol, Ciprofloxacin and Norfloxacin.
