CAJ | 학술논문

目的了解HIV-1毒株亚型在深圳地区吸毒人群中的流行情况,并分析其流行趋势及进化规律.方法收集1996-2008年深圳地区吸毒人群HIV确认阳性样本166例,应用巢式聚合酶链反应(nPCR)技术,对样本膜蛋白基因(env)进行扩增,并对其基因区核苷酸序列进行测定和分析.结果 166份样本中共存在CRF01_AE、CRF07_BC、CRF08_BC3种重组毒株以及B′、C、A13种亚型;其在所有分析样本中的比例分别为65.06%(108/166)、19.88%(33/166)、6.02%(10/166)、7.23%(12/166)、0.60%(1/166)和1.20%(2/166);系统进化分析发现,不同时间段HIV-1各亚型出现较明显的汇聚现象;3个不同时间段所流行的CRF01_AE、CRF07_BC亚型组内及与标准参考株间的基因离散率有较为显著性的增大趋势.结论深圳地区吸毒人群HIV-1流行毒株以CRF01_AE重组亚型为主,其次是CRF07_BC重组亚型,也存在CRF08_BC、B′、C、A1亚型,并且各亚型在流行过程中已发生较大变异.
목적 료해HIV-1독주아형재심수지구흡독인군중적류행정황,병분석기류행추세급진화규률.방법 수집1996-2008년심수지구흡독인군HIV학인양성양본166례,응용소식취합매련반응(nPCR)기술,대양본막단백기인(env)진행확증,병대기기인구핵감산서렬진행측정화분석.결과 166빈양본중공존재CRF01_AE、CRF07_BC、CRF08_BC3충중조독주이급B′、C、A13충아형;기재소유분석양본중적비례분별위65.06%(108/166)、19.88%(33/166)、6.02%(10/166)、7.23%(12/166)、0.60%(1/166)화1.20%(2/166);계통진화분석발현,불동시간단HIV-1각아형출현교명현적회취현상;3개불동시간단소류행적CRF01_AE、CRF07_BC아형조내급여표준삼고주간적기인리산솔유교위현저성적증대추세.결론 심수지구흡독인군HIV-1류행독주이CRF01_AE중조아형위주,기차시CRF07_BC중조아형,야존재CRF08_BC、B′、C、A1아형,병차각아형재류행과정중이발생교대변이.
Objective To study the prevalent status of human immunodeficiency virus-1 (HIV-1) subtypes in IDU (injecting drug users) population in Shenzhen and to study their source of infection in order to predict the epidemic trend and evolution. Methods 166 HIV-1 positive plasma from the IDUs was collected from 1996 to 2008. HIV-1 env genes were amplified by nested-PCR from RNA. The C2-V3 regions (450 bp) of HIV-1 env were sequenced for analyses. Phylogenetic analyses were performed on the nucleotide sequence data. Results Among 166 samples, there were 6 HIV-1 strains including CRF01_AE, CRF08_BC, CRF07_BC 3 circulating recombinant forms (CRFs) and B',C, A1 3 subtypes. Data from the genotype analyses showed that 65.06% (108/166) were CRF01_AE, 19.88% (33/166) were CRF07 BC_6.02% (10/166) were CRF08_BC, 7.23%(9/166) were subtype B', 0.60% (1/166) were subtype C and 1.20% (2/166) were subtype A1. Phylogenetic tree analysis showed that some of HIV-1 clusters defined in CRF01_AE, CRF07_BC and subtype B' in different time groups. Significant increase of gene distance in CRF01_AE and CRF07_BC strains in the three different periods. Conclusion CRF01_AE and CRF07_BC were the major epidemic CRF strains among the IDU population in Shenzhen while the subtype B', C, A1 and CRF08_BC were also circulating in IDU population in this region. The variation of all different subtypes was increasing through these years.