CAJ | 학술논문

目的探讨肝癌肝移植病人移植前后外周血和肿瘤组织中CD4+CD25+FOXP3+T细胞比例变化及其临床意义.方法用流式细胞仪检测肝癌肝移植病人和其他肝移植病人术后外周血中CD4+CD25+FOXP3+T细胞的比例,并采用正常人作对照.用免疫组化法检测肝癌病人和非肝癌病人肿瘤组织中FoxP3的表达及CD8+T细胞浸润的比例.观察肝癌肝移植病人术后及肿瘤复发后调节性T细胞的变化及其对肿瘤复发的影响.结果流式细胞检测显示肝癌肝移植、非肝癌肝移植的病人术后外周血中CD4+CD25+FOXP3+T细胞占CD4+T细胞的比例较正常人明显升高,分别为(10.15±1.00)%、(5.30±1.64)%和(3.20±1.18)%,P<0.05.肝癌肝移植肿瘤复发病人较未复发病人外周血CD4+CD25+FOXP3+T比例明显升高,分别为(15.15±1.50)%和(6.80±1.50)%,P<0.01.免疫组化检测显示肿瘤组织中FOXP3+T细胞增多,CD8+T细胞浸润明显减少.结论肝癌肝移植肿瘤复发的病人外周血中CD4+CD25+FOXP3+T细胞比例升高.调节性T细胞可能通过减少CD8+T细胞浸润,加速肿瘤复发.
목적 탐토간암간이식병인이식전후외주혈화종류조직중CD4+CD25+FOXP3+T세포비례변화급기림상의의.방법 용류식세포의검측간암간이식병인화기타간이식병인술후외주혈중CD4+CD25+FOXP3+T세포적비례,병채용정상인작대조.용면역조화법검측간암병인화비간암병인종류조직중FoxP3적표체급CD8+T세포침윤적비례.관찰간암간이식병인술후급종류복발후조절성T세포적변화급기대종류복발적영향.결과 류식세포검측현시간암간이식、비간암간이식적병인술후외주혈중CD4+CD25+FOXP3+T세포점CD4+T세포적비례교정상인명현승고,분별위(10.15±1.00)%、(5.30±1.64)%화(3.20±1.18)%,P<0.05.간암간이식종류복발병인교미복발병인외주혈CD4+CD25+FOXP3+T비례명현승고,분별위(15.15±1.50)%화(6.80±1.50)%,P<0.01.면역조화검측현시종류조직중FOXP3+T세포증다,CD8+T세포침윤명현감소.결론 간암간이식종류복발적병인외주혈중CD4+CD25+FOXP3+T세포비례승고.조절성T세포가능통과감소CD8+T세포침윤,가속종류복발.
Objective To investigate the impact of CD4+ CD25+ FOXP3+ regulatory T(Treg) cells on tumor recurrence in liver transplantation for hepatocellular carcinoma (HCC). Methods Im-munohistochemistry and flow cytometry were used for analysis of the frequency of Treg. Meanwhile,it was compared with that of non-cancer liver transplantation patients. Results The frequency of CD4+CD25+ FOXP3+ regulatory T cells in the blood of HCC liver transplantation was (10. 15 ±1. 00) % , which was significantly higher than that in the normal control group (3. 20±1. 18) %. Cir-culating CD4+ CD25+ FOXP3+ Treg frequency was increased significantly and correlated with the tumor recurrence in the HCC patients. An abundant accumulation of Treg concurrent with significant-ly reduced infiltration of CD8+T cells was found in tumor regions. Conclusion Increased CD4+ D25+FoxP3+ Treg may impair the effectors function of CD8+ T cells, promote the tumor recurrence and re-present a therapeutic target for HCC liver transplantation.