中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
6期
343-347
,共5页
苏跃青%伍严安%陈发林%黄肖莉%吴小青%曾秀雅
囌躍青%伍嚴安%陳髮林%黃肖莉%吳小青%曾秀雅
소약청%오엄안%진발림%황초리%오소청%증수아
呼吸道感染%肺类病毒属%呼吸道合胞体病毒,人%反转录聚合酶链反应%基因型
呼吸道感染%肺類病毒屬%呼吸道閤胞體病毒,人%反轉錄聚閤酶鏈反應%基因型
호흡도감염%폐류병독속%호흡도합포체병독,인%반전록취합매련반응%기인형
Respiratory tract infections%Pneumovirus%Respiratory syncytial virus,human%Reverse transcriptase polymerase chain reaction%Genotype
目的 了解福州地区人类偏肺病毒(HMPV)感染情况,比较HMPV与呼吸道合胞病毒(RSV)引起呼吸道感染的临床特征及流行特点.方法 采集2005年至2007年连续两个冬春季节153份福建省立医院就诊呼吸道感染患者痰标本或咽拭子标本,RT-PCR和套式RT-PCR分别检测RSV和HMPV,部分阳性PCR产物测序,DNAMAN软件分析;结合临床资料,比较两种病毒所引起的呼吸道感染临床症状、体征和流行特点.结果 153份鼻咽分泌物标本中,32份HMPV阳性,阳性率为20.9%;26份RSV阳性,阳性率为17.0%,8份HMPV和RSV均阳性.随机抽取3份标本,HMPV核苷酸序列一致,登载NCBI基因库(序列号DQ887758),基因进化树分析为单一基因型,属A基因型,但发生部分变异.2005年至2006年冬春HMPV阳性26份,阳性率为26.7%,2006年至2007年冬春HMPV阳性6份,阳性率为10.7%,而RSV检出情况与HMPV相反.儿童RSV感染平均年龄为(2.65±2.65)岁,HMPV为(4.58±3.35)岁.两种病毒引起呼吸道感染症状均以咳嗽、咽痛,发热为主.结论 HMPV与RSV均是福州地区冬春季节呼吸道病毒感染的主要病原体,两者可合并感染;HMPV主要感染年龄较大儿童,HMPV与RSV的临床特征相似.本研究期间福州地区发现的HMPV为单一基因型.
目的 瞭解福州地區人類偏肺病毒(HMPV)感染情況,比較HMPV與呼吸道閤胞病毒(RSV)引起呼吸道感染的臨床特徵及流行特點.方法 採集2005年至2007年連續兩箇鼕春季節153份福建省立醫院就診呼吸道感染患者痰標本或嚥拭子標本,RT-PCR和套式RT-PCR分彆檢測RSV和HMPV,部分暘性PCR產物測序,DNAMAN軟件分析;結閤臨床資料,比較兩種病毒所引起的呼吸道感染臨床癥狀、體徵和流行特點.結果 153份鼻嚥分泌物標本中,32份HMPV暘性,暘性率為20.9%;26份RSV暘性,暘性率為17.0%,8份HMPV和RSV均暘性.隨機抽取3份標本,HMPV覈苷痠序列一緻,登載NCBI基因庫(序列號DQ887758),基因進化樹分析為單一基因型,屬A基因型,但髮生部分變異.2005年至2006年鼕春HMPV暘性26份,暘性率為26.7%,2006年至2007年鼕春HMPV暘性6份,暘性率為10.7%,而RSV檢齣情況與HMPV相反.兒童RSV感染平均年齡為(2.65±2.65)歲,HMPV為(4.58±3.35)歲.兩種病毒引起呼吸道感染癥狀均以咳嗽、嚥痛,髮熱為主.結論 HMPV與RSV均是福州地區鼕春季節呼吸道病毒感染的主要病原體,兩者可閤併感染;HMPV主要感染年齡較大兒童,HMPV與RSV的臨床特徵相似.本研究期間福州地區髮現的HMPV為單一基因型.
목적 료해복주지구인류편폐병독(HMPV)감염정황,비교HMPV여호흡도합포병독(RSV)인기호흡도감염적림상특정급류행특점.방법 채집2005년지2007년련속량개동춘계절153빈복건성립의원취진호흡도감염환자담표본혹인식자표본,RT-PCR화투식RT-PCR분별검측RSV화HMPV,부분양성PCR산물측서,DNAMAN연건분석;결합림상자료,비교량충병독소인기적호흡도감염림상증상、체정화류행특점.결과 153빈비인분비물표본중,32빈HMPV양성,양성솔위20.9%;26빈RSV양성,양성솔위17.0%,8빈HMPV화RSV균양성.수궤추취3빈표본,HMPV핵감산서렬일치,등재NCBI기인고(서렬호DQ887758),기인진화수분석위단일기인형,속A기인형,단발생부분변이.2005년지2006년동춘HMPV양성26빈,양성솔위26.7%,2006년지2007년동춘HMPV양성6빈,양성솔위10.7%,이RSV검출정황여HMPV상반.인동RSV감염평균년령위(2.65±2.65)세,HMPV위(4.58±3.35)세.량충병독인기호흡도감염증상균이해수、인통,발열위주.결론 HMPV여RSV균시복주지구동춘계절호흡도병독감염적주요병원체,량자가합병감염;HMPV주요감염년령교대인동,HMPV여RSV적림상특정상사.본연구기간복주지구발현적HMPV위단일기인형.
Objective To investigate the prevalence of human metapneumovirus (HMPV)infection in patients with respiratory infection in Fuzhou area and compare their epidemic features and clinical characteristics with those of infected with respiratory syncytial virus (RSV). Methods A total of 153 sputum or pharyngeal swab samples from patients with respiratory tract infection were collected in Fujian Provincial Hospital in consecutive winter and spring seasons from 2005 to 2007. HMPV was detected by nested reverse transcriptase-polymerase chain reaction (RT-PCR) and RSV was tested by RT-PCR. Parts of PCR products were sequenced and analyzed using DNAMAN software. The clinical symptoms, signs and epidemiology of the respiratory tract infections caused by HMPV and RSV were compared. Results In the 153 specimens, 32 (20.9%) were positive for H MPV, 26 (17.0%) were positive for RSV, and 8 were both HMPV and RSV positive. Nucleotide sequences of three 432-bp PCR products were 100% identical and submitted to GenBank (the accession No. DQ887758).Phylogenetic tree analysis of nucleotide sequences revealed that the three isolates clustered in HMPV belonged to genotype A with part of mutation. Twenty-six samples (26. 7%) were HMPV positive from Dec 2005 to Apr 2006 and 6 (10.7%) were positive from Dec 2006 to Apr 2007. The RSV detection rate was opposite of HMPV. The mean age of RSV infection in children was (2.65±2.65)years old and HMPV infection was (4.58 ±3.35) years old. The main clinical manifestations of both RSV and HMPV infections were cough, sore throat and fever. Conclusions Both HMPV and RSV are the major pathogens of respiratory tract infection in Fuzhou area and HMPV and RSV coinfection could be detected. HMPV infected children are older. The clinical features of HMPV and RSV infections are similar. Single genotype of HMPV is detected in Fuzhou area in this study.