陕西师范大学学报：自然科学版
陝西師範大學學報：自然科學版
협서사범대학학보：자연과학판
Journal of Shaanxi Normal University： Nat Sci Ed
2011年
4期
60-64
,共5页
一氧化氮%一氧化氮合酶%绿豆插条%萘乙酸%不定根形成
一氧化氮%一氧化氮閤酶%綠豆插條%萘乙痠%不定根形成
일양화담%일양화담합매%록두삽조%내을산%불정근형성
nitric oxide%nitric oxide synthase%mung bean cuttings%NAA%adventitious root formation
研究了新型信号分子一氧化氮（NO）在萘乙酸（NAA）诱导绿豆下胚轴插条不定根形成过程中的作用.结果表明,与对照相比,一氧化氮供体硝普钠（SNP）和NAA都显著诱导插条不定根形成,根数、鲜重和干重都显著高于对照.SNP和NAA复合处理的生根效果明显好于两者单独处理.不同浓度NO清除剂羧基-2-苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物（c-PTIO）能够有效抑制NAA诱导不定根形成,其最适浓度为200μmol/L.此外,一氧化氮合酶（NOS）抑制剂L-硝基精氨酸甲酯（L-NAME）也能够部分抑制NAA诱导的生根效应,最适浓度为30mmol/L.内源NO检测结果表明NAA处理后NO水平明显提高,c-PTIO和L-NAME则抑制此效应.结果表明,NO在NAA诱导绿豆下胚轴插条不定根形成过程中起着至关重要的作用,NOS参与此过程中NO的产生.
研究瞭新型信號分子一氧化氮（NO）在萘乙痠（NAA）誘導綠豆下胚軸插條不定根形成過程中的作用.結果錶明,與對照相比,一氧化氮供體硝普鈉（SNP）和NAA都顯著誘導插條不定根形成,根數、鮮重和榦重都顯著高于對照.SNP和NAA複閤處理的生根效果明顯好于兩者單獨處理.不同濃度NO清除劑羧基-2-苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物（c-PTIO）能夠有效抑製NAA誘導不定根形成,其最適濃度為200μmol/L.此外,一氧化氮閤酶（NOS）抑製劑L-硝基精氨痠甲酯（L-NAME）也能夠部分抑製NAA誘導的生根效應,最適濃度為30mmol/L.內源NO檢測結果錶明NAA處理後NO水平明顯提高,c-PTIO和L-NAME則抑製此效應.結果錶明,NO在NAA誘導綠豆下胚軸插條不定根形成過程中起著至關重要的作用,NOS參與此過程中NO的產生.
연구료신형신호분자일양화담（NO）재내을산（NAA）유도록두하배축삽조불정근형성과정중적작용.결과표명,여대조상비,일양화담공체초보납（SNP）화NAA도현저유도삽조불정근형성,근수、선중화간중도현저고우대조.SNP화NAA복합처리적생근효과명현호우량자단독처리.불동농도NO청제제최기-2-분-4,4,5,5-사갑기미서-1-양-3-양화물（c-PTIO）능구유효억제NAA유도불정근형성,기최괄농도위200μmol/L.차외,일양화담합매（NOS）억제제L-초기정안산갑지（L-NAME）야능구부분억제NAA유도적생근효응,최괄농도위30mmol/L.내원NO검측결과표명NAA처리후NO수평명현제고,c-PTIO화L-NAME칙억제차효응.결과표명,NO재NAA유도록두하배축삽조불정근형성과정중기착지관중요적작용,NOS삼여차과정중NO적산생.
The role of nitric oxide（NO） in naphthaleneacetic acid（NAA）-induced adventitious roots formation in mung bean（Phaseolus radiatus L.） hypocotyl cuttings was studied.The results showed that,compared with the control,both NO donor sodium nitroprusside（SNP） and NAA significantly raised number,fresh and dry weight of adventitious roots.The treatment with complex of NAA and SNP resulted in significant increase in adventitious roots number compared with the treatment using SNP or NAA alone.Carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline（c-PTIO）,a NO specific scavenger,effectively inhibited NAA-induced adventitious root formation,and the optimal concentration was 200 μmol/L.Furthermore,inhibitor of nitric oxide synthase（NOS）,N＇-nitro-L-arginine methyl ester（L-NAME） partly inhibited the effect from NAA,the optimal concentration was 30mmol/L.Detection of endogenous NO showed that the level of NO was obviously promoted by NAA,however,innibitd by c-PTIO and L-NAME.All results mentioned above indicated that NO plays a key role in NAA-induced adventitious roots formation,and NOS is involved in the generation of NO in this process.
