CAJ | 학술논문

2009年5月,贵州省贵定县人工饲养的大鲵(娃娃鱼)发生了以四肢和腹侧的皮肤溃烂、口腔粘膜弥漫性出血以及肝脏肿大为临床病理特征的传染病.本研究对该传染病进行了病原分离、动物回归、菌株16SrRNA基因测序检测、药物敏感性、药物治疗及灭活乳化疫苗免疫保护方面的实验研究.试验结果表明,动物回归分离菌株与病原分离菌株其形态特征及理化特性一致,分离菌基因16S rRNA测序检测与嗜水气单胞菌的同源性达到99.57%以上,因此确诊该病为鱼类嗜水气单孢菌感染.致病株具有较强的耐药性,敏感药物治疗能抗菌,但皮肤溃烂组织、深部肌肉组织抗炎效果较差.经致病菌株灭活乳化免疫和免疫保护攻毒试验表明,灭活乳化疫苗免疫平均保护率达83.33%.
2009년5월,귀주성귀정현인공사양적대예(왜왜어)발생료이사지화복측적피부궤란、구강점막미만성출혈이급간장종대위림상병리특정적전염병.본연구대해전염병진행료병원분리、동물회귀、균주16SrRNA기인측서검측、약물민감성、약물치료급멸활유화역묘면역보호방면적실험연구.시험결과표명,동물회귀분리균주여병원분리균주기형태특정급이화특성일치,분리균기인16S rRNA측서검측여기수기단포균적동원성체도99.57%이상,인차학진해병위어류기수기단포균감염.치병주구유교강적내약성,민감약물치료능항균,단피부궤란조직、심부기육조직항염효과교차.경치병균주멸활유화면역화면역보호공독시험표명,멸활유화역묘면역평균보호솔체83.33%.
In May 2009, a communicable disease was detected in hand-feeding giant salamander with the pathology characteristics of ulceration skin on theirs extremities and ventral, theirs oral mucosa hemorrhage and liver tumescence that the disease was discovered in Guiding county, Guizhou Province. Then we carried out some empirical study with the disease, such as pathogeny isolation, recurrence of animals, analysis of 16S rRNA sequence of the isolated strain, drug sensitivity, drug treatment and protection of inactivated emulsified vaccine series of experiments. The results showed that both the morphological character and physiological functions of the strains isolated from recurrence animals was the same as the strains isolated from pathogeny, and the homology of 16S rRNA sequence between the isolated strain and A eromonas hydrophila was above 99.57%. Accordingly, the final diagnosis of the disease was infection of Aeromonas hydrophila. It was also found that pathogenic strains had the strong drug resistance, and drug treatment showed that sensitive medication had antimicrobial function for therapy, nevertheless, the anti-inflammatory effect was less function in ulceration skin organizations and deep muscle tissue. The protection of inactivated emulsified vaccine series of experiments revealed that the average rate of inactivated and emulsified vaccine protection was 83.33%.