CAJ | 학술논문

本文研究了鸟苷酸二钠(GMPNa_2)对羟自由基和DPPH·自由基的清除效果,同时建立了H_2O_2氧化损伤体外培养脾细胞模型,用MTT法检测GMPNa_2的修复及增殖作用,并分析了GMPNa_2对细胞抗氧化体系及抗氧化能力的影响,以评价其抗氧化活性.结果表明:GMPNa_2具有剂量依赖性的体外抗氧化和清除活性氧能力,10 mmol/L GMPNa_2的羟自由基清除能力高达96.644%,但DPPH清除率为6.589%.GMPNa_2清除羟自由基与同浓度Vc相近,而其清除DPPH自由基的能力很弱.添加0.5、1、5和10 mmol/L GMPNa_2均能显著修复H_2O_2诱导的脾细胞氧化损伤(P<0.05),提高总抗氧化能力和抗氧化酶类活力(P<0.01).GMPNa_2添加量大于1mmol/L时,可显著降低MDA含量(P<0.01).说明GMPNa_2具有显著的抗氧化作用.
본문연구료조감산이납(GMPNa_2)대간자유기화DPPH·자유기적청제효과,동시건립료H_2O_2양화손상체외배양비세포모형,용MTT법검측GMPNa_2적수복급증식작용,병분석료GMPNa_2대세포항양화체계급항양화능력적영향,이평개기항양화활성.결과표명:GMPNa_2구유제량의뢰성적체외항양화화청제활성양능력,10 mmol/L GMPNa_2적간자유기청제능력고체96.644%,단DPPH청제솔위6.589%.GMPNa_2청제간자유기여동농도Vc상근,이기청제DPPH자유기적능력흔약.첨가0.5、1、5화10 mmol/L GMPNa_2균능현저수복H_2O_2유도적비세포양화손상(P<0.05),제고총항양화능력화항양화매류활력(P<0.01).GMPNa_2첨가량대우1mmol/L시,가현저강저MDA함량(P<0.01).설명GMPNa_2구유현저적항양화작용.
Tbe antioxidant activities of GMPNa_2 on hydroxyl free radical(·OH) and DPPH free radical(DPPH·) were studied by chemical colorimetry. The splenic cells of mice were treated with H_2O_2 to cause oxidative stress injury and they were determined by MTT method. To investigate in vitro antioxidant effect of GMPNa_2 on repairing of injury, total antioxidation capability (T-AOC) , catalase (CAT) activities, glutathione peroxidase (GSH-Px) activities and diaminodi-phenyl methane(MDA) in ineubation medium were detected. The results indicated that GMPNa_2 Could scavenge free rad-icals in concentration dependent manner in vitro and significantly repair the injury of H_2O_2 in splenic cells. GMPNa_2 at a concentration of 10 mmol/L showed 96. 644% inhibition ratio to hydroxyl free radical,and only 6. 589% to DPPH. The hydroxyl free radical scavenging capacity of GMPNa_2 was nearly to that of Vc. Compared with the injured group ,the num-ber of live cells increased signifieandy in H_2O_2 injured splenic cells, with the supplementation of 0.5,1,5 and 10 mmol/L GMPNa_2 (P < 0.05). CAT activities, GSH-Px activities and T-AOC in incubation medium were increased significantly with GMPNa_2 concentration increased (P < 0.01). MDA content decreased significantly (P < 0.01) with supplementation of more than 1 mmol/L GMPNa_2 to the injured group. It was concluded that GMPNa_2 has the significant anti-oxidation activity.