CAJ | 학술논문

应用23个形态学特征,19个扩增片段长度多态性(AFLP)引物组合,80个随机扩增多态DNA(RAPD)引物和32个简单序列重复(SSR)引物对,比较三种分子标记法在29个杏仁栽培种和3个野生种遗传关系构建中的信息景和效率.根据预期杂合度的评价,与AFLPs和RAPDs相比,SSRs具有较高水平的多态性和较大的信息量.AFLPs预期朵合度值最低,但其辨别效率值最高,因为AFLPs能揭示每个反应中的大量条带,导致各种类型的多样性指数均较高.三种分子标记法对杏仁基因型的辨别效率均较高,只是SSRs无法辨别‘Monagha'和‘Sefied'杏仁基因型.三种分子标记法基因型相似性相关系数统计上显著,但SSR数据要低于RAPDs和AFLPs的值.尽管三种分子标记法树形图拓扑结构存在一些差异,但相似性水平均较高.SSRs、RAPDs和AFLPs的系统树图及其综合数据都能依据地理散布反映大多数栽培种的关系.AMOVA检测到每个地理组中栽培种和野生种的变异.辅助程序分析表明,实验所应用的标记物数量足以保证基因相似性估计的可靠性和标记法间的比较是有意义的.
응용23개형태학특정,19개확증편단장도다태성(AFLP)인물조합,80개수궤확증다태DNA(RAPD)인물화32개간단서렬중복(SSR)인물대,비교삼충분자표기법재29개행인재배충화3개야생충유전관계구건중적신식경화효솔.근거예기잡합도적평개,여AFLPs화RAPDs상비,SSRs구유교고수평적다태성화교대적신식량.AFLPs예기타합도치최저,단기변별효솔치최고,인위AFLPs능게시매개반응중적대량조대,도치각충류형적다양성지수균교고.삼충분자표기법대행인기인형적변별효솔균교고,지시SSRs무법변별‘Monagha'화‘Sefied'행인기인형.삼충분자표기법기인형상사성상관계수통계상현저,단SSR수거요저우RAPDs화AFLPs적치.진관삼충분자표기법수형도탁복결구존재일사차이,단상사성수평균교고.SSRs、RAPDs화AFLPs적계통수도급기종합수거도능의거지리산포반영대다수재배충적관계.AMOVA검측도매개지리조중재배충화야생충적변이.보조정서분석표명,실험소응용적표기물수량족이보증기인상사성고계적가고성화표기법간적비교시유의의적.
A total 23 morphological traits, 19 AFLP-primer combinations, 80 RAPD primers and 32 SSR primer pair were used to compare the informativeness and efficiency of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers in establishing genetic relationships among 29 almond culfivars and three related wild species. SSRs presented a high level of polymorphism and greater information content, as assessed by the expected hetrozygosity, compared to AFLPs and RAPDs. The lowest values of expected hetrozygosity were obtained for AFLPs; however AFLPs showed the highest efficiency, owing to their capacity to reveal large numbers of bands per reaction, which led to high values for various types of indices of diversity. All the three techniques discriminated almond gcnotypes very effectively, except that SSRs failed to discriminate between 'Monagha' and 'Sefied' al-mond genotypes. The correlation coefficients of similarity were statistically significant for all the three marker systems, but wore lower for the SSR data than for RAPDs and AFLPs. For all the markers, high similarity in dcndrogram topologies was obtained, although some dif-ferences were observed. All the dendrograms, including that obtained by the combined use of all the marker data, reflect relationships for 1most of cyltivars according to their geographic diffusion. AMOVA detected more variation among cultivated and related wild species of al-mond within each geographic group. Bootstrap analysis revealed that the number of markers used was sufficient for reliable estimation of genetic similarity and for meaningful comparisons of marker types.