CAJ | 학술논문

应用蛋白质组学、电镜观察、脂质过氧化物测定等方法研究了两个不同类型水稻品种的根系对有机污染物1,2,4-三氯苯(TCB)胁迫的应答.结果表明,TCB胁迫抑制水稻根系生长,影响根细胞结构,导致脂质过氧化.TCB胁迫对水稻根系蛋白质组也有显著影响.TCB胁迫能诱导根系内不同类型蛋白质的表达,它们是:解毒酶(包括脂酶、醛/酮还原酶、谷胱甘肽-硫转移酶),细胞壁物质代谢相关酶(包括蛋白转葡萄糖基酶、GDP-甘露糖-3,5-异构酶1),激素代谢或调节相关酶(包括酸式-还原酮二加氧酶、β-葡萄糖苷酶、病程相关蛋白家族10中的2个不同蛋白),原初或次生代谢相关酶(包括转录延长因子、细胞质磷酸丙酮酸双激酶、磷酸丙糖异构酶、丙氨酸氨基转移酶和异黄酮还原酶).TCB胁迫诱导矮仔占根系中β-葡萄糖苷酶和病程相关蛋白家族10蛋白的表达,及汕优63根系中谷胱甘肽-S-转移酶和酸式-还原酮二加氧酶的表达,这种诱导蛋白的差异可能是汕优63较矮仔占对TCB胁迫有较高耐性的机理之一.
응용단백질조학、전경관찰、지질과양화물측정등방법연구료량개불동류형수도품충적근계대유궤오염물1,2,4-삼록분(TCB)협박적응답.결과표명,TCB협박억제수도근계생장,영향근세포결구,도치지질과양화.TCB협박대수도근계단백질조야유현저영향.TCB협박능유도근계내불동류형단백질적표체,타문시:해독매(포괄지매、철/동환원매、곡광감태-류전이매),세포벽물질대사상관매(포괄단백전포도당기매、GDP-감로당-3,5-이구매1),격소대사혹조절상관매(포괄산식-환원동이가양매、β-포도당감매、병정상관단백가족10중적2개불동단백),원초혹차생대사상관매(포괄전록연장인자、세포질린산병동산쌍격매、린산병당이구매、병안산안기전이매화이황동환원매).TCB협박유도왜자점근계중β-포도당감매화병정상관단백가족10단백적표체,급산우63근계중곡광감태-S-전이매화산식-환원동이가양매적표체,저충유도단백적차이가능시산우63교왜자점대TCB협박유교고내성적궤리지일.
The responses of rice roots to 1,2,4-trichlorobenzene (TCB) stress were investigated by proteomic analysis, electron micrograph observation, and lipid peroxide estimation. The results showed that TCB stress inhibited root growth, led to water deficit in rice seedlings, affected cell structure and caused lipid peroxidation in rice roots. Moreover, TCB stress had significant effect on global proteome in rice roots. The analysis of the category and function of TCB stress inducible proteins showed that different groups of proteins were induced by 5 mg L-1 TCB stress. They are detoxification enzymes (including esterase, aldo/keto reductases, and glutathione S-transferase), cell wall compound metabolism related enzymes (including UDP-glucose protein transglucosylase and GDP-mannose 3,5-epimerase 1), phytohormone metabolism and regulation related enzymes or proteins (including aci-reductone dioxygenase 4, beta-glucosidase, two members of pathogenesis-related proteins from family 10), primary and secondary metabolism regulative enzymes (including translational elongation factor Tu, cytosolic orthophosphate dikinase, triosephosphate isomerases, alanine aminotransferase, and isoflavone reductase). Compared the TCB stress inducible proteins between two cultivars, β-glucosidase and PR10 proteins were particularly induced in Aizizhan roots, and glutathione S-transferase and aci-reductone dioxygenase 4 were induced in Shanyou 63 roots. This might be one of the important mechanisms for Shanyou 63 having higher tolerance to TCB stress than Aizizhan.