CAJ | 학술논문

用一种已知的抗表皮生长因子受体抑制剂 (piceatannol) 作为半抗原与载体牛血清白蛋白 (BSA) 连接后免疫制备相应的多克隆抗体 (PcAb).利用该多克隆抗体来模拟酶制成亲和色谱柱,从一种藏药粗提物中将包括该半抗原在内的几种结构不同的抗表皮生长因子受体抑制剂识别出来 .研究采用前沿亲和色谱质谱联用技术对样品进行分析,可以直接从中药复杂体系中识别出有效成分并进行鉴定,实现中药有效成分的筛选与鉴定一体化技术 .
용일충이지적항표피생장인자수체억제제 (piceatannol) 작위반항원여재체우혈청백단백 (BSA) 련접후면역제비상응적다극륭항체 (PcAb).이용해다극륭항체래모의매제성친화색보주,종일충장약조제물중장포괄해반항원재내적궤충결구불동적항표피생장인자수체억제제식별출래 .연구채용전연친화색보질보련용기술대양품진행분석,가이직접종중약복잡체계중식별출유효성분병진행감정,실현중약유효성분적사선여감정일체화기술 .
A polyclonal antibody (PcAb) prepared with piceatannol,a known inhibitor against the epidermal growth factor receptor (EGFR),was adopted as a stationary phase in the chromatographic system.Using the antibody to mimic the enzyme,several anti-EGFR inhibitors were recognized directly from a crude extract of Tibetan herb.Frontal affinity chromatography (FAC) was used here for analyzing molecular interactions between the analytes and an immobilized ligand (in this case the PcAb conjugated to Sepharose CL-4B) by calculating the extent of retardation of the elution front.By combining FAC with mass spectrometry (MS) in the current study,a very efficient and straightforward procedure was developed for analyzing the binding properties of different inhibitors.The novel effective screening of anti-EGFR inhibitors using PcAb from natural resources affords us a new feasible approach for the discovery of lead compounds.