CAJ | 학술논문

目的:为在脂质体介导基因转染人低分化鼻咽癌上皮细胞株CNE-2Z时获得较高转染效率,同时观察GFP作为报告基因用于脂质体转染条件优化的可行性。方法:以GFP为报告基因,通过比较碱裂解法与PEG纯化法制备质粒;改变DNA、脂质体量及转染时间,在荧光显微镜下观察并计算转染效率。结果:在35 mm培养皿中,2 μ gDNA与4 μ L脂质体转染CNE-2Z细胞8 h,转染效率最高,且在显微镜下观察不到细胞的形态变化。结论:在35 mm培养皿中,2 μ gDNA与4 μ L脂质体转染CNE-2Z细胞8 h,可获得最佳转染效率,GFP可作为报告基因优化脂质体转染条件
목적:위재지질체개도기인전염인저분화비인암상피세포주CNE-2Z시획득교고전염효솔,동시관찰GFP작위보고기인용우지질체전염조건우화적가행성。방법:이GFP위보고기인,통과비교감렬해법여PEG순화법제비질립;개변DNA、지질체량급전염시간,재형광현미경하관찰병계산전염효솔。결과:재35 mm배양명중,2 μ gDNA여4 μ L지질체전염CNE-2Z세포8 h,전염효솔최고,차재현미경하관찰불도세포적형태변화。결론:재35 mm배양명중,2 μ gDNA여4 μ L지질체전염CNE-2Z세포8 h,가획득최가전염효솔,GFP가작위보고기인우화지질체전염조건
Objective:To obtain higher efficiency in liposome-mediated gene transferinto CNE-2Z cells.Methods:Transfection efficiencies at different doses of DNA,liposome and transfection time were calculated under fluorescent microscopy using GFP (Green Fluorescent Protein)as a reporter gene.Results:In 35 mm cell culture plate,2 μg DNA with 4μL liposome resulted in the highest transfection efficiency and the morphology of　CNE-2Z cell can not saw by using microscopy after 8 h transfection. Conclusion:The experiment implied result show that　GFP a convenient reporter gene.