遗传
遺傳
유전
HEREDITAS(BEIJING)
2001年
2期
107-110
,共4页
黄代新%张林%吴梅筠%陈国弟%陈于波
黃代新%張林%吳梅筠%陳國弟%陳于波
황대신%장림%오매균%진국제%진우파
短串联重复序列%聚合酶链反应%D7S2201基因座%多态性
短串聯重複序列%聚閤酶鏈反應%D7S2201基因座%多態性
단천련중복서렬%취합매련반응%D7S2201기인좌%다태성
用扩增片段长度多态性技术分析短串联重复序列D7S2201基因座的遗传多态性,在262个中国成都地区汉族无关个体及119个泰国曼谷地区泰人无关个体中分别发现7个和5个等位基因,首次获得该基因座在两群体中的频率分布,其等位基因片段大小范围为100~124bp。两群体的基因型频率分布均符合Hardy Weinberg平衡。该基因座在两群体中的个人识别能力(PD)、杂合度(H)、多态性信息含量(CPI)及非父排除率(PE)分别为0.7038、0.5992、0.4789、0.2900和0.7351、0.5882、0.5012、0.2770。家系调查证实了等位基因的传递遵循孟德尔遗传规律。χ2检验表明两群体间等位基因频率分布无显著性差异。
用擴增片段長度多態性技術分析短串聯重複序列D7S2201基因座的遺傳多態性,在262箇中國成都地區漢族無關箇體及119箇泰國曼穀地區泰人無關箇體中分彆髮現7箇和5箇等位基因,首次穫得該基因座在兩群體中的頻率分佈,其等位基因片段大小範圍為100~124bp。兩群體的基因型頻率分佈均符閤Hardy Weinberg平衡。該基因座在兩群體中的箇人識彆能力(PD)、雜閤度(H)、多態性信息含量(CPI)及非父排除率(PE)分彆為0.7038、0.5992、0.4789、0.2900和0.7351、0.5882、0.5012、0.2770。傢繫調查證實瞭等位基因的傳遞遵循孟德爾遺傳規律。χ2檢驗錶明兩群體間等位基因頻率分佈無顯著性差異。
용확증편단장도다태성기술분석단천련중복서렬D7S2201기인좌적유전다태성,재262개중국성도지구한족무관개체급119개태국만곡지구태인무관개체중분별발현7개화5개등위기인,수차획득해기인좌재량군체중적빈솔분포,기등위기인편단대소범위위100~124bp。량군체적기인형빈솔분포균부합Hardy Weinberg평형。해기인좌재량군체중적개인식별능력(PD)、잡합도(H)、다태성신식함량(CPI)급비부배제솔(PE)분별위0.7038、0.5992、0.4789、0.2900화0.7351、0.5882、0.5012、0.2770。가계조사증실료등위기인적전체준순맹덕이유전규률。χ2검험표명량군체간등위기인빈솔분포무현저성차이。
The polymorphism of a new short tandem repeat (STR) locus D7S2201 was analyzed by using AmpFLP. Seven alleles were observed in 262 unrelated Chinese individuals living in Chengdu and five alleles in 119 unrelated Thai individuals living in Bangkok, the ranges of fragment size were 100~124bp. The genotypes distributions of D7S2201 locus in the two populations were in accordance with Hardy Weinberg equilibrium. The discriminating power (PD), observed heterozygosity (H), polymorphism information content (CPI) and power of exclusion (PE) were 0.7038, 05992, 04789, 02900 in Chinese population and 0.7351, 0.5882, 0.5012, 0.2770 in Thai population respectively. Family studies confirmed Mendelian inheritance of alleles. No significant difference was observed between the two populations.