CAJ | 학술논문

目的从人胎盘组织制备促进免疫细胞增殖提取物并寻求保存提取物的适宜条件。方法通过热处理胎盘匀浆液制备提取物，并用溴化四唑蓝染料结合法测定体外促小鼠脾淋巴细胞的增殖活性，同时对提取物进行放射性同位素60Co照射。结果经Lowry′s法测定为每1 g胎盘组织含2～3mg蛋白质，其促细胞增殖率＞80%，经同位素处理后低温保存数月活性不受影响。结论此法制备的提取物不仅活性高，且方法简便、重复性好，该提取物作为一种稳定、可靠、明显的促淋巴细胞增殖反应具有上规模开发、生产、应用于临床的价值。
목적종인태반조직제비촉진면역세포증식제취물병심구보존제취물적괄의조건。방법통과열처리태반균장액제비제취물，병용추화사서람염료결합법측정체외촉소서비림파세포적증식활성，동시대제취물진행방사성동위소60Co조사。결과경Lowry′s법측정위매1 g태반조직함2～3mg단백질，기촉세포증식솔＞80%，경동위소처리후저온보존수월활성불수영향。결론차법제비적제취물불부활성고，차방법간편、중복성호，해제취물작위일충은정、가고、명현적촉림파세포증식반응구유상규모개발、생산、응용우림상적개치。
Purpose　The aim is to prepare the extract of immunocompetent cell proliferation from human placenta and to try to find out a suitable method of preserving the extract.Methods　The extract was mainly prepared by heat-treating placenta homogenated fluid. Then the activity to stimulate murine splenic lymphocyte proliferation in vitro was done with MTT , exposing　the　extract to　radioisotope 60　Co.Results　The content of protein was 2～3mg per gram placenta measured by Lowry′s Method.The rate to promote cell proliferation was more than 80 percent.The activity lasted a few months after being exposed to radioisotope.Conclusion　The extract prepared by heat-treating not only had high activity, but also had the unique method and good repeatability.This prepared extract as a kind of stable,reliable and remarkably promoting lymphocyte proliferation reaction had the value of development production on broad scale as well as in clinical practice.