生物技术通讯
生物技術通訊
생물기술통신
LETTERS IN BIOTECHNOLOGY
2001年
2期
99-102
,共4页
张丽旌%任邦彦%李平忠%李桂兰%李国良
張麗旌%任邦彥%李平忠%李桂蘭%李國良
장려정%임방언%리평충%리계란%리국량
培养方法%Vero细胞%评价
培養方法%Vero細胞%評價
배양방법%Vero세포%평개
对三种不同培养方法进行细胞生长速度、密度、营养及代谢产物浓度的比较分析，以优化和筛选最佳培养条件与方式。用同体积生物反应罐，基本培养条件相同，采用批培养、再循环培养、灌流培养三种方式进行了Vero细胞微载体（Cytodex I）的周期培养。三种培养方法均达到预期效果，最终细胞密度分别为每毫升2.09×106、3.37×106、4.34×106。灌流培养倍增水平最高(5.59)，倍增时间最少(30.05 h)。表明灌流培养优于再循环培养；而再循环培养兼容批培养及灌流培养的特点，又优于批培养。
對三種不同培養方法進行細胞生長速度、密度、營養及代謝產物濃度的比較分析，以優化和篩選最佳培養條件與方式。用同體積生物反應罐，基本培養條件相同，採用批培養、再循環培養、灌流培養三種方式進行瞭Vero細胞微載體（Cytodex I）的週期培養。三種培養方法均達到預期效果，最終細胞密度分彆為每毫升2.09×106、3.37×106、4.34×106。灌流培養倍增水平最高(5.59)，倍增時間最少(30.05 h)。錶明灌流培養優于再循環培養；而再循環培養兼容批培養及灌流培養的特點，又優于批培養。
대삼충불동배양방법진행세포생장속도、밀도、영양급대사산물농도적비교분석，이우화화사선최가배양조건여방식。용동체적생물반응관，기본배양조건상동，채용비배양、재순배배양、관류배양삼충방식진행료Vero세포미재체（Cytodex I）적주기배양。삼충배양방법균체도예기효과，최종세포밀도분별위매호승2.09×106、3.37×106、4.34×106。관류배양배증수평최고(5.59)，배증시간최소(30.05 h)。표명관류배양우우재순배배양；이재순배배양겸용비배양급관류배양적특점，우우우비배양。
Vero cells were cultivated periodically on microcarriers Cytodex I by using batch culture mode, recirculation culture mode and perfusion culture mode, respectively in the bioreactor of the same volume 7L and under the basic cultivation conditions. Expected result were obtained for three culture modes. Their final cell densities were: 2.09×106 cells/ml; 3.37×106 cells/ml; 4.34×106cells/ml; while the perfusion cultures doubling level was the highest of 5.59; the doubling time was the least of 30.05 hour. The experimental data indicated that the perfusion culture, was excelled than that of the recirculation culture, and that the recirculation culture which had the characteristics of both perfusion and batch cultures was excelled than that of the batch culture.
