CAJ | 학술논문

目的探讨皮肌炎皮损、肌细胞及浸润淋巴细胞凋亡的表达及皮质类固醇激素的影响。方法对22个标本用TUNEL原位末端标记法检测角质形成细胞、肌细胞及淋巴细胞凋亡。10例用皮质激素者为A组，12例未用者为B组。结果 A组角质形成细胞、肌细胞及淋巴细胞有较多的凋亡，凋亡指数（AI）明显大于B组（P<0.05）。皮质类固醇激素使用时间在2周以内者，凋亡细胞较少。在观察中未发现凋亡与皮肤、肌肉病理损害程度有关。结论皮质类固醇激素可能具有诱导角质形成细胞、肌细胞及淋巴细胞凋亡的作用，这可能是皮质类固醇激素的治疗机理之一。激素使用时间的长短似与细胞凋亡程度有关。
목적탐토피기염피손、기세포급침윤림파세포조망적표체급피질류고순격소적영향。방법대22개표본용TUNEL원위말단표기법검측각질형성세포、기세포급림파세포조망。10례용피질격소자위A조，12례미용자위B조。결과 A조각질형성세포、기세포급림파세포유교다적조망，조망지수（AI）명현대우B조（P<0.05）。피질류고순격소사용시간재2주이내자，조망세포교소。재관찰중미발현조망여피부、기육병리손해정도유관。결론피질류고순격소가능구유유도각질형성세포、기세포급림파세포조망적작용，저가능시피질류고순격소적치료궤리지일。격소사용시간적장단사여세포조망정도유관。
Objective　This study inquired into the relationship between the influence of corticosteroid and the expression of apoptosis，Fas and Bcl-2 in muscle from patients with dermatomyositis（DM）.Methods　Corticosteroid was given to group A (10 DM cases) but not given to group B (12 DM cases). Results　Apoptotic cells were detected in situ by terminal deoxynucleotidyl transferase-mediated-dUTP nick end labeling (TUNEL). Fas and Bcl-2 expressions was determined with the use of immunohistochemical staining in DM. The results showed that more expression of apoptosis in keratinocytes，muscle cells and lymphocytes were observed in group A. The apoptotic index (AI) of muscle cells and that of lymphocytes were more marked in group A than in group B (P<0.05). Less apoptosis cells were observed in two weeks of treatment, and no correlation was found between apoptosis and the lesion of skin and muscle. Conclusion　The authors suggest that corticosteroid may play a role in inducing the apoptosis of keratinocytes，muscle cells and lymphocytes，which may be one of the therapeutic mechanisms of corticosteroid. The length of administering corticosteroid to the patients seems related to apoptosis.