CAJ | 학술논문

目的：探讨β2-糖蛋白Ⅰ(β2-GPⅠ)在体外的氧化修饰，为其在体内诱导抗磷脂抗体产生研究作准备。方法：首先纯化并鉴定大鼠β2-GPⅠ，然后在体外以次黄嘌呤/黄嘌呤氧化酶氧化系统氧化大鼠β2-GPⅠ。结果：大鼠的β2-GPⅠ在体外被氧化产生羰基，氧化后的羰基含量与氧化前的比较有显著差异(P<0.05)。结论：在体外β2-GPⅠ可以被次黄嘌呤/黄嘌呤氧化酶氧化系统氧化产生羰基。
목적：탐토β2-당단백Ⅰ(β2-GPⅠ)재체외적양화수식，위기재체내유도항린지항체 산생연구작준비。방법：수선순화병감정대서β2-GPⅠ，연후재체외이차황표령/황표령양화매양화계 통양화대서β2-GPⅠ。결과：대서적β2-GPⅠ재체외피양화산생탄기，양화후적탄기함량여양화전적비교유현 저차이(P<0.05)。 결론：재체외β2-GPⅠ가이피차황표령/황표령양화매양화계통양화산생탄기。
Objective:To investigate oxidative modification of β2-glycoprotein Ⅰ in vit ro.Methods:Rat β2-glycoprotein Ⅰ was purified and characterized,then oxidize d by hypoxanthine plus xanthine oxidase as a supreroxide free radical generating system;carbonl groups of β2-glycoprotein Ⅰ were detected by the reaction w ith 2,4-dinitrophenylhudrazine.Results:There was a significant increase of carbonyl groups formation in β2- glycoprotein Ⅰ oxidized in comparison with native β2-glycoprotein Ⅰ (P <0.05). Conclusion:Carbonyl groups have been formed in vitro on rat β2-glycoprotein Ⅰ after oxidative modification using hypoxanthine plus xanthine oxidase system.