CAJ | 학술논문

探讨中药复方止消通脉宁对糖尿病大鼠肾脏一氧化氮合成酶(NOS)基因表达的影响。采用链脲佐菌素(STZ)腹腔注射建立模型, 分为模型组、中药治疗组、西药治疗组、正常对照组,并应用反转录多聚酶链反应技术(RT-PCR), 观察NOSmRNA在各组间的表达情况。结果：与正常对照组相比,模型组肾脏诱生型NOS(iNOS)表达明显增高,中药及西药均可使iNOS表达水平下调, 中药组下调更为明显。
탐토중약복방지소통맥저대당뇨병대서신장일양화담합성매(NOS)기인표체적영향。채용련뇨좌균소(STZ)복강주사건립모형, 분위모형조、중약치료조、서약치료조、정상대조조,병응용반전록다취매련반응기술(RT-PCR), 관찰NOSmRNA재각조간적표체정황。결과：여정상대조조상비,모형조신장유생형NOS(iNOS)표체명현증고,중약급서약균가사iNOS표체수평하조, 중약조하조경위명현。
The effects of the traditional Chinese preparation, Zhixiaotongm aining (ZTN), on the gene expression of iNOS mRNA in the kidney were studied in rats w ith experimental diabetes. The rats were divided into the model (established by abdominal injection of streptozotocin) group, ZTN treating group, general drug t reating group and control group, and the RT-PCR technique was used to observe th e expression of iNOS mRNA in all the four groups. The results showed that compar ed with the control group, the level of the expression of iNOS mRNA was obviousl y higher in the model group, that in the ZTN treating group or the general drug treating group was lower, especially that in the ZTN treating group.