CAJ | 학술논문

目的研究地塞米松（Dxs）对肺纤维化大鼠肺单核细胞趋化蛋白-1（MCP-1）mRNA表达的影响，阐明糖皮质激素治疗肺纤维化疾病的分子机制。方法气管内滴注博莱霉素致大鼠肺纤维化模型，不同浓度的地塞米松处理后，观察肺组织的病理学变化和计数5×5 mm高倍视野内炎症细胞数目;RT-PCR方法检测肺组织MCP-1 mRNA表达水平。结果地塞米松减少了肺组织炎症细胞的聚集，延缓了肺纤维化的形成过程，并抑制了MCP-1 mRNA的表达，呈现一定的剂量依赖效应趋势。结论地塞米松治疗肺纤维化疾病的分子机制与抑制肺MCP-1基因表达有关。
목적연구지새미송（Dxs）대폐섬유화대서폐단핵세포추화단백-1（MCP-1）mRNA표체적영향，천명당피질격소치료폐섬유화질병적분자궤제。방법기관내적주박래매소치대서폐섬유화모형，불동농도적지새미송처리후，관찰폐조직적병이학변화화계수5×5 mm고배시야내염증세포수목;RT-PCR방법검측폐조직MCP-1 mRNA표체수평。결과지새미송감소료폐조직염증세포적취집，연완료폐섬유화적형성과정，병억제료MCP-1 mRNA적표체，정현일정적제량의뢰효응추세。결론지새미송치료폐섬유화질병적분자궤제여억제폐MCP-1기인표체유관。
AIM　To study the effects of dexamethasone on expressing monocyte chemoattractant protein-1(MCP-1 ) mRNA in the rats with pulmonary fibrosis, elaborate the molecular mechanism of dexamethasone (Dxs) in pulmonary fibrosis therapy. METHODS　The model of pulmonary fibrosis was established by instilling bleomycin intratracheally. After treating with Dxsip, the levels of MCP-1 mRNA were determined by RT-PCR. The histological changes were observed and the numbers of inflammatory cells were counted in optical microscopy field. RESULTS　The accumulation of inflammatory cells decreased markedly, and the symptom of pulmonary fibrosis was alleviated. Furthermore, Dxs evidently inhibited the expression of MCP-1 mRNA in lung tissues with pulmonary fibrosis. CONCLUSION　The molecular mechanism of Dxs in pulmonary fibrosis therapy was associated with inhibiting the expression of MCP-1 mRNA.