CAJ | 학술논문

【目的】以地高辛（DIG）标记探针检测汉族肥胖者瘦素（ leptin）基因的表达。【方法】以DIG用随机引物法标记瘦素cDNA片段，以此探针，采用斑点杂交方法检测11例汉族正常体重者(体重指数，MBI为21.0 kg/m2±1.5 kg/m2)和 12例汉族肥胖者(MBI为28.5 kg/m2±2.3 kg/m2)腹部皮下脂肪组织瘦素 mRNA的表达水平。【结果】 DIG leptin cDNA探针可检出5 pg的pUC-leptin DNA，与pUC19 DNA无杂交信号。肥胖组腹部皮下脂肪组织瘦素mRNA相对含量明显高于对照组（312.8±108.9 vs 175.9±81.5，P<0.05）；且与BMI呈正相关（r=0.56，P<0.05）。【结论】肥胖者脂肪组织瘦素mRNA水平明显增高，且与BMI呈正相关。
【목적】 이지고신（DIG）표기탐침검측한족비반자수소（ leptin）기인적표체。【방법】 이DIG용수궤인물법표기수소cDNA편단，이차탐침，채용 반점잡교방법검측11례한족정상체중자(체중지수，MBI위21.0 kg/m2±1.5 kg/m2)화 12례한족비반자(MBI위28.5 kg/m2±2.3 kg/m2)복부피하지방조직수소 mRNA적표체 수평。【결과】 DIG leptin cDNA탐침가검출5 pg적pUC-leptin DNA，여pUC19 DNA무잡교 신호。비반조복부피하지방조직수소mRNA상대함량명현고우대조조（312.8±108.9 vs 175.9±81.5，P<0.05）；차여BMI정정상관（r=0.56，P<0.05）。【결론】 비반자지방조직수소mRNA수평명현증고，차여BMI정정상관。
【Objective】 To study the expression of leptin mRNA in Chinese obese subjects using dot blot hybridization with a digoxigenin labele d probe. 【Methods】 11 Chinese nonobese subjects (bodymass index, BMI 21.0 kg/ m2±1.5 kg/m2) and 12 Chinese obese subjects(BMI 28.5 kg/m2±2.3 kg/m 2) parti cipated in the study. The human leptin cDNA probe was labeled wih digoxigenin(DI G) b y the random priming method. Expression of leptin mRNA in abdominal adipose tiss ue has been examined using dot blot hybridization with this probe. 【Results】 T he expression of leptin mRNA was significantly higher in obese subjects than in non-obese ones (312.8±108.9 vs 175.9±81.5, relative units, P<0.0 5), an d correlated with BMI (r=0.56, P<0.05). 【Conclusions】 Leptin mRNA le vel is high in obese subjects, and correlated with BMI.